摘要
We describe a rapid, inexpensive and scalable hybrid bioseparation technique for one-step purification of humanized monoclonal antibodies from mammalian cell culture supernatant. It involves the selective and reversible retention of the monoclonal antibody within a membrane module utilizing a combination of microfiltration of precipitated antibody and simultaneous hydrophobic interaction-based membrane chromatography of soluble antibody on the same microfiltration membrane. The retained monoclonal antibody is subsequently recovered from the membrane module in a highly pure form by changing the solution condition to that which favours simultaneous antibody dissolution and elution from the membrane. This combination of separation principles results in high-capacity, high-resolution separation. The monoclonal antibody purities and recoveries obtained in the experiments described in this paper were very high.
摘要译文
我们描述了一种快速，廉价和可扩展的混合生物分离技术，用于从哺乳动物细胞培养上清液一步纯化人源化单克隆抗体。它涉及在同一微滤膜上利用沉淀抗体的微滤和同时基于疏水相互作用的可溶性抗体的膜层析的组合在膜组件内选择性和可逆地保留单克隆抗体。随后通过将溶液条件改变为有利于同时从膜上溶解和洗脱的溶液条件，以高度纯的形式从膜组件中回收保留的单克隆抗体。这种分离原理的组合导致高容量，高分辨率的分离。本文所描述的实验中得到的单克隆抗体纯度和回收率非常高。
LuWang;Dharmesh M.Kanani;RajaGhosh;. Purification of humanized monoclonal antibodies by membrane-based hybrid bioseparation technique[J]. Journal of Immunological Methods, 2006,314(1-2): 1-8