摘要
Modulation of hippocampal synaptic plasticity by androgen has been attracting much attention. Thorns of thorny excrescences of CA3 hippocampal neurons are post-synaptic regions whose presynaptic partners are mossy fiber terminals. Here we demonstrated rapid effects of dihydrotestosterone (DHT) and testosterone (T) on the density of thorns, by imaging Lucifer Yellow-injected neurons in adult male rat hippocampal slices. The application of 10 nM DHT or T induced rapid increase in the density of thorns within 2 h. The androgen-mediated increase was suppressed by blocking several kinases, such as Erk MAPK, p38 MAPK, PKC, and CaMKII. On the other hand, PKA, PI3K were not involved in the signaling of thorn-genesis. The increase in the thorn density by androgen was also blocked by the inhibitor of classical androgen receptor. Almost no difference was observed between DHT and T in the effect on the thorn density. We observed that the androgen-induced thorn-genesis is opposite to estrogen-induced thorn-degeneration.
摘要译文
通过雄激素调节海马突触可塑性一直受到很多关注。CA3海马神经元棘突的荆棘是突触前的伴侣是苔藓纤维终端的突触后突触区。在这里，通过在成年雄性大鼠海马切片中成像Lucifer黄色注射的神经元，我们展示了二氢睾酮（DHT）和睾酮（T）对荆棘密度的快速影响。10 nM DHT或T的应用诱导荆棘密度在2 h内快速增加。雄激素介导的增加通过阻断几种激酶（例如Erk MAPK，p38MAPK，PKC和CaMKII。另一方面，PKA，PI3K没有参与刺激的信号传导。雄激素刺激密度的增加也被经典雄激素受体抑制剂阻断。 DHT和T之间几乎没有观察到对刺刺密度的影响。我们观察到，雄激素诱导的刺形成与雌激素诱导的刺变性相反。
Yusuke Hatanaka[a][c]; Hideo Mukai[a][b][c]; Kenji Mitsuhashi[a][c]; Yasushi Hojo[a][b][c]; Gen Murakami[a][c]; Yoshimasa Komatsuzaki[a]; Rei Sato[a][c]; Suguru Kawato[a][b][c]. Androgen rapidly increases dendritic thorns of CA3 neurons in male rat hippocampus[J]. Biochemical and Biophysical Research Communications, 2009,381(4): 728–732