摘要
The objective of this study, was to compare the developmental competence of bovine in vitro fertilized embryos in three different culture methods; microdrop method (50 μL of medium under mineral oil in petri dishes) compared to tube methods (1 mL of medium in tubes) with or without oil overlay and to examine the influence of Fetal Bovine Serum (FBS) in culture methods. They were randomly allocated to one of following culture conditions: (Group 1) microdrop method supplemented with 10% (v:v) FBS at day 5 post-insemination (pi); (Group 2) microdrop method without FBS; (Group 3) tube method with oil overlay and FBS added at day 5 pi; (Group 4) tube method with oil overlay; (Group 5) tube method without oil overlay but FBS added at day 5 pi; (Group 6) tube method without oil overlay and FBS. There were no differences (p>0.05) in cleavage rates among the culture methods (78.3, 75.6 and 74.9% for microdrop, tube with oil overlay and tube method without overlay, respectively). However, regardless of serum addition, blastocyst rates in the microdrop method (30.5%) were significantly higher (p<0.05) than those in the tube method without oil overlay (9.8%). There was no difference (p>0.05) between with or without serum in blastocyst rate regardless culture methods. Numerically, the highest blastocyst rate was observed in the microdrop method with the FBS supplementation (Group 1; 36.6%). Overall, the microdrop method was the optimum culture method among the culture methods; however, serum supplementation did not significantly affect the blatocyst rate.
摘要译文
本研究的目的，是比较在三种不同的培养方法体外受精胚胎牛的发育能力;下的矿物油中的培养皿）相比，管的方法（1毫升介质中管）有或无油覆盖并审查胎牛血清（FBS）的培养方法的影响平台。它们被随机分配到以下培养条件之一：（1组）微滴的方法补充有10％（体积：体积）FBS的在后第5天授精（PI）; （第2组）无FBS微滴的方法;（第3组）管法与石油覆盖，并添加FBS在每天5 PI; （4组）管法与石油覆盖; （5组）无油覆盖，但FBS管法加入5天PI;（6组）管法无油叠加和FBS。目前还没有差异（p 0.05）卵裂率之间的培养方法（78.3，75.6和74.9％的微滴，管与油叠加和管法无重叠，分别）。然而，不管血清。另外，囊胚率在微滴法（30.5％）是显著升高（P 0。05）高于无油覆盖（9.8％）的管法。有有或无血清中囊胚率，无论培养方法之间没有差异（P 0.05）。数字，最高囊胚率中观察到的微滴的方法与FBS补充（组1; 36.6％）。总体而言，微滴法是其中的培养方法的最佳培养方法;然而，血清补充没有显著影响blatocyst速率。
Song-yi Jung and Scott T. Willard. The Effect of Culture Methods and Serum Supplementation on Developmental Competence of Bovine Embryos Cultured In vitro[J]. Journal of Animal and Veterinary Advances, 2009,8(1): 67-74