摘要
The present study was conducted to examine the fertility of ewes inseminated intrauterinally with frozen semen using semen extender containing either egg yolk or bovine serum albumin (BSA). Sixty Suffolk and cross-bred ewes were treated with controlled internal drug release (CIDR) devices during the non-breeding season (July 2006). A CIDR was inserted into the vagina for 12 days and an intramuscular injection of 500 IU equine chorionic gonadotropin was administered one day before its removal. Ejaculates from a suffolk ram were diluted with a Tris-based extender containing either 15% (v/v) egg yolk or 10% (w/v) BSA, and the diluted semen was frozen in 0.25 ml straws. A fixed-time intrauterine artificial insemination (AI) was performed 43-47 h after CIDR removal, regardless of incidence of estrus. There was no significant difference in pregnancy rates at 60 days after AI between the extenders containing egg yolk (66.7%, 20/30 animals) or BSA (65.5%, 19/29 animals). Furthermore, there were no significant difference in the lambing rates (66.7% and 62.1%) and prolificacy (1.25 and 1.56) between the two semen extenders. The present study indicates that a semi-defined semen extender containing 10% BSA produces fertility after intrauterine AI that is similar to that achieved with semen extender containing egg yolk.
摘要译文
本研究的目的是检查母羊与使用含有任一蛋黄或牛血清白蛋白（BSA）精液剂冷冻精液授精intrauterinally的生育能力。六十萨福克杂交母羊治疗与非繁殖季节控制的内部药物释放（CIDR）设备（2006年7月）。甲CIDR被插入阴道12天，注射500 IU马绒毛膜促性腺激素肌内其去除之前施用一天。从萨福克公羊精液稀释用的Tris-基于增量含有要么15％（体积/体积）蛋黄或10％（重量/体积）BSA和稀释精液冷冻于0.25ml吸管。固定时间宫腔内人工授精（AI）进行的，无论发情的发病率43-47ħCIDR移除后，。还有60天，怀孕率不显著差异AI后，含蛋黄（66.7％，20/30动物）或BSA（65.5％，19/29动物）的扩展器之间。此外，有两个精液扩展的产羔率（66.7％和62.1％）和繁殖力（1.25和1.56）没有显著差异。本研究表明，含有10％BSA的半定义精液剂产生生育宫内的AI之后，它类似于该实现与含有精液扩蛋黄。
Yutaka FUKUI[1]; Hirohide KOHNO[2]; Tetsuro TOGARI[3];Mami HIWASA[1]. Fertility of Ewes Inseminated Intrauterinally with Frozen Semen Using Extender Containing Bovine Serum Albumin[J]. Journal of Reproduction and Development, 2007,53(4): 959-962