摘要
Background
Leptospirosis is an emerging infectious disease. The differential diagnosis of leptospirosis is difficult due to the varied and often "flu like" symptoms which may result in a missed or delayed diagnosis. There are over 230 known serovars in the genus Leptospira. Confirmatory serological diagnosis of leptospirosis is usually made using the microscopic agglutination test (MAT) which relies on the use of live cultures as the source of antigen, often performed using a panel of antigens representative of local serovars. Other techniques, such as the enzyme linked immunosorbent assay (ELISA) and slide agglutination test (SAT), can detect different classes of antibody but may be subject to false positive reactions and require confirmation of these results by the MAT.
摘要译文
背景钩端螺旋体病是一种新兴传染病。钩端螺旋体病的鉴别诊断是困难的，因为各种各样的并且通常“流感样”症状可能导致错过或延迟的诊断。已知的钩端螺旋体属230多种血清型。钩端螺旋体病的确诊血清学诊断通常使用依靠使用活培养物作为抗原来源的显微凝集试验（MAT）来进行，通常使用一组代表局部血清型的抗原进行。其他技术，如酶联免疫吸附试验（ELISA）和玻片凝集试验（SAT），可以检测不同种类的抗体，但可能会受到假阳性反应，需要通过MAT确认这些结果。
Lee D Smythe [1] Ina L Smith [2] Greg A Smith [2] Michael F Dohnt [1] Meegan L Symonds [1] Leonie J Barnett [1] David B McKay [3]]. A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp[J]. BMC Infectious Diseases, 2002,2(1)