摘要
Partial sequences of the mitochondrial genomes were determined for two calanoid copepods, Eucalanus bungii [9530 bp (base pairs)] and Neocalanus cristatus (7965 bp), using an approach that employs a long polymerase chain reaction (PCR) technique and primer walking. In contrast to Tigriopus japonicus (Harpacticoida), in which the complete mitochondrial genome has been determined, genes are encoded on both strands of the genome. In spite of the close relationship between E. bungii and N. cristatus, the gene orders of these calanoid copepods differed greatly from each other. From the sequence we obtained, new copepod-specific versatile primers were designed for the cytochrome c oxidase I (COI) gene (1200 bp). These primer pairs, as well as the previously designed primer pairs for the small ribosomal RNA (srRNA) gene (500 bp), were applied for 20 species representing four orders of both calanoid and non-calanoid copepods and amplification of sequences from at least one species from each order was successful.
摘要译文
确定了两种卡那类桡足类Eucalanus bungii [9530bp（碱基对）]和Neocalanus cristatus（7965bp）的线粒体基因组的部分序列，使用采用长聚合酶链反应（PCR）技术和引物步行的方法。与天竺葵（Harpactoida）相反，其中已经确定完整的线粒体基因组，基因被编码在基因组的两条链上。尽管E. bungii和N. cristatus之间有密切的关系，这些胼these体的基因序列彼此差异很大。从我们获得的序列，为细胞色素c氧化酶I（COI）基因（1200 bp）设计了新的桡足类特异性通用引物。这些引物对，以及先前设计的小核糖体RNA（srRNA）基因（500bp）的引物对，应用于20种物种，代表四个类别的卡那样和非卡那类桡足类，并且从每个顺序的至少一个物种的序列的扩增成功。
Ryuji J Machida[a]; Masaki U Miya[b]; Mutsumi Nishida[a]; Shuhei Nishida[a]. Large-scale gene rearrangements in the mitochondrial genomes of two calanoid copepods Eucalanus bungii and Neocalanus cristatus (Crustacea), with notes on new versatile primers for the srRNA and COI genes[J]. Gene, 2004,332: 71–78