摘要
Transcervical insemination (TCI) has generated recent interest as an assisted reproductive technique in the dog. A case series study was performed to determine if TCI using frozen–thawed semen was a viable technique to offer in a general veterinary practice setting. Over a period exceeding 28 months, 137 Greyhound bitches were presented for assisted breeding. A single, timed insemination using a rigid cystoscope to aid in transcervical deposition of a frozen–thawed semen dose was given within 72 h after the behaviorally estrual bitch had a >4 ng/mL serum progesterone concentration and estrus-categorized vaginal cytology. Litter size, pregnancy and whelping rate were collected; their association to semen center and stud dog were quantified. Of the 137 bitches, 117 were bred for one cycle and 20 were bred for two or more cycles, giving a total of 161 single, timed inseminations. Pregnancy rate was 89.4%, with 141 (87.5%) whelping. Litter size was 6.9 ± 2.7 (mean ± S.D.) pups. Semen center (P = 0.84) and stud (P = 0.79) had no effect on pregnancy. These results were quite favorable when compared to prior TCI studies, and are possibly due to the use of a single breed (i.e., Greyhound) with good fertility. This study supported the application of TCI, in Greyhounds, as a successful and viable service to offer in private practice. Additionally, these results have value in their use for benchmarking future breed-specific and TCI research. Serendipitously, the apparent fecundity results obtained in this observational study suggests a possible greater appreciation be given to breed composition and choice in assisted reproductive technique studies.
摘要译文
经宫颈人工授精（TCI）近来引起了狗的兴趣，作为辅助生殖技术。进行病例系列研究以确定使用冷冻 - 解冻精液的TCI是否是在一般兽医实践环境中提供的可行技术。在超过28个月的时间内，提供了137只灰狗母犬用于辅助育种。一个单一的，在行为上雌性婊子具有 4ng / mL血清孕酮浓度和发情分类的阴道细胞学后72小时内，冷冻 - 解冻精液剂量的子宫颈沉积被给予。收集窝窝大小，怀孕和育雏率;他们与精液中心和狗狗的关系被量化。在137个母狗中，117个育成了一个周期，20个育种了两个或更多个周期，总计161个单独的定时授精。妊娠率为89.4％，141（87.5％）例。窝大小为6.9±2。7（平均值±S.D.）幼仔。精液中心（P \x3d 0.84）和钉（P \x3d 0.79）对怀孕没有影响。与之前的TCI研究相比，这些结果是相当有利的，并且可能是由于使用具有良好繁殖力的单一品种（即灰狗）。这项研究支持TCI在赛狗中的应用，作为在私人执业中提供的成功和可行的服务。此外，这些结果在将其用于基准未来品种特异性和TCI研究方面具有价值。无心插柳，在这个观察性研究中获得的明显的繁殖力结果表明可能更好的鉴定品种组成和在辅助生殖技术研究中的选择。
S.D.Pretzer[a];R.K.Lillich[a];G.C.Althouse[b];. Single, transcervical insemination using frozen–thawed semen in the Greyhound: A case series study[J]. Theriogenology, 2006,65(6): 1029-1036