摘要
Acid-sensing ion channels (ASICs) are ligand-gated cation channels that respond to acidic stimuli. They are expressed throughout the mammalian nervous system. In the peripheral nervous system, ASICs act as nociceptors, responding to the tissue acidosis that accompanies ischemic and inflammatory conditions. The function of ASICs in the central nervous system is not known. In this article, the authors present evidence that transient global ischemia induces ASIC 2a protein expression in neurons that survive ischemia. Western blot analysis with an anti-ASIC 2a antibody revealed up-regulation of an 80 kD protein in ischemic rat brain. Immunohistochemical analysis showed that ASIC 2a protein expression increased in neurons of the hippocampus and cortex. Klenow fragment-mediated labeling of DNA strand breaks determined that ASIC 2a induction did not occur in cells with detectable DNA damage. The current results suggest a possible role for ASICs in mediating a cellular response to ischemia.
摘要译文
酸感应离子通道（ASICs）是响应于酸性刺激的配体门控阳离子通道。它们在整个哺乳动物神经系统中表达。在周围神经系统中，ASIC作为伤害感受器，对伴随缺血性和炎性疾病的组织酸中毒作出反应。 ASIC在中枢神经系统中的功能尚不清楚。在这篇文章中，作者提供证据表明瞬时全球缺血诱导在缺血后存活的神经元中的ASIC 2a蛋白表达。使用抗ASIC 2a抗体的Western印迹分析显示缺血性大鼠脑中80kD蛋白的上调。免疫组织化学分析显示，ASIC 2a蛋白表达在海马和皮层神经元中增加。 Klenow片段介导的DNA链断裂的标记确定ASIC 2a诱导不发生在具有可检测DNA损伤的细胞中。目前的结果表明ASIC可能在介导细胞对缺血的反应方面发挥作用。
Martha B. Johnson. Global Ischemia Induces Expression of Acid-Sensing Ion Channel 2a in Rat Brain[J]. Journal of Cerebral Blood Flow & Metabolism, 2001,21(6): 734-740