摘要
Background
Early diagnosis of leptospirosis is important for ensuring better clinical management and achieving better outcomes. Currently, serological assays suffer from inconsistent performance and are less useful for early diagnosis of leptospirosis. As an alternative, qPCR is more sensitive, specific and able to detect the presence of leptospiral DNA during the acute phase of the infection. Meanwhile, most molecular assays do not detect the non-pathogenic group of Leptospira, even though these groups may also infect humans, although less frequently and less severely.
摘要译文
背景钩端螺旋体病的早期诊断对于确保更好的临床管理和取得更好的结果非常重要。目前，血清学分析的表现不一致，对于早期诊断钩端螺旋体病的用处不大。作为替代，qPCR更敏感，特异并且能够在感染的急性阶段检测到钩端螺旋体DNA的存在。同时，大多数分子检测方法检测不到钩端螺旋体的非致病性组，即使这些组也可能感染人类，尽管不太频繁且不太严重。
Mohammad RidhuanMohd Ali[a][b];Amira WahidaMohd Safee[a];Nor HayatiIsmail[c];RoslindaAbu Sapian[b];HaniMat Hussin[c];NabilahIsmail[a];ChanYean Yean[a][d];. Development and validation of pan-Leptospira Taqman qPCR for the detection of Leptospira spp. in clinical specimens[J]. Molecular and Cellular Probes, 2018,38: 1-6