摘要
The ability to mature and fertilize oocytes of endangered species may allow us to sustain genetic and global biodiversity. Epididymis sperms may be the last chance to ensure preservation of genetic materials after injury or death of a valuable animal. Studies have been conducted to determine wether both epididymis sperms and oocytes can be used to produce viable embryos and offspring. The purpose of this study was to determine how long cats sperms contained in epididymis were remain motile and had intact membranes when preserved at 4 oC, and to determine whether such those preserved sperms are able to fertilize oocytes. Epididymis was preserved immediately in phosphate buffer saline at 4 oC for 1, 3, and 6 days. The observation of sperm quality and viability after preservation was performed by vital staining acrosom and Hoechst-Propidium Iodine. Biological functions of sperms were evaluated by in vitro culture technique for fertilization, micro fertilization and embryonic development rate in CR1aa medium. The results showed that average motility of sperms collected from ductus deferens, cauda and corpus epididymis decreased not significantly (P > 0.05) from 0, 1, 3, and 6 days of preservation times (from 83.0%, 80.2%, 79.0%; 80.9%, 75.0%, 75.5%; 52.0%, 63.2%, 55.0% to 34.6%, 34.6%, 33.3%, respectively). The general results showed that sperms from epididymis preserved for 1, 3, and 6 days can be used for IVF. The rate of embryonal cleavage produced by IVF technique using sperms collected from epididymis preserved for 1-, 3- and 6-days were 33.3, 26.7, and 20.0%, respectively and significantly different (P < 0.05) from that of controll (50.0%). In conclusion, sperms contained in epididyimis preserved at 4 oC in PBS (Phospate Buffer Saline) for 1-6 days can be used to IVF and in vitro production of cat embryos.
摘要译文
能力成熟和受精濒危物种的卵母细胞可以使我们保持遗传和全球生物多样性。附睾精子可能是最后的机会，以保证受伤了宝贵的动物或死亡后保存遗传物质。研究已进行，以确定阉既附睾精子和卵母细胞可用于产生可行的胚胎和后代。本研究的目的是确定包含在附睾精子多久猫均保持能动并具有完整的膜时在4℃保存，并确定这样的那些保存精子是否能够受精的卵母细胞。附睾立即被保存在磷酸盐缓冲盐水中，在4摄氏度为1,3，和6天。由活体染色acrosom和赫斯特 - 碘化丙啶进行精子的质量和活力的保存后的观察。精子生物学功能体外受精，受精微型和胚胎发育率CR1aa中培养技术进行了评估。结果表明，精子从输精管收集平均蠕动，马尾和胼附睾降低不显著性（P 0.05），从0，1，3，6天的保存时间（从83.0％，80.2％，79.0％; 80.9％，75.0％，75.5％; 52.0％，63.2％，55.0％至34.6％，34.6％，分别为33.3％）。在一般的结果表明，从附睾精子保存1，3，6天，可用于体外受精。通过使用从附睾收集的精子体外受精技术产生胚胎裂解保存1-，3-和6-天率分别为33.3，26.7和20.0％，分别从控制研究（50.0％）的显著差异（P 0.05）。结论，载于epididyimis于PBS（磷酸酯缓冲盐水）为1-6天在4℃保存精子可用于IVF和体外生产猫胚胎。
KARTINI ERIANI ; ARIEF BOEDIONO ; ITA DJUWITA ; SONY HERU SUMARSONO ; AL-AZHAR4. Development of Domestic Cat Embryo Produced by Preserved Sperms[J]. HAYATI Journal of Biosciences, 2008,15(4): 155-160