摘要
Excystment experiments were carried out on cysts of Alexandrium minutum and A. tamarense (Group III) taken from Cork Harbour, Ireland. Freshly sampled cysts were isolated into well plates and their excystment was monitored over a 30 day period. A pronounced seasonality was observed in the excystment behaviour in both species when measurements were carried out seasonally. Between 80 and 100% of the isolated cysts excysted within 30 days when samples were taken between March and June. However, between only 0 and 15% excysted in samples taken between August and early February. This seasonal characteristic was observed repeatedly over a 3 year period (2004–2007). The effect was observed in cysts which had been sampled from both inter-tidal and sub-tidal locations. No endogenous clock was evident in the germination of A. tamarense and A. minutum cysts taken from Cork Harbour which had been stored cool and in the dark under anoxic conditions for up to 18 months. The seasonal effect observed was independent of water temperature, although temperature did affect the rate of excystment. Temperature also affected the maturation of cysts which had been freshly formed in the laboratory. The significance of incorporating seasonality in excystment of Alexandrium into models describing its growth is also discussed.
摘要译文
对来自爱尔兰科克港的小亚历山大藻和塔玛亚历山大藻（第三组）的孢囊进行了实验。将新鲜采样的囊肿分离到孔板中，并在30天内监测它们的胞吐。季节性进行测量时，观察到两种物种的脱囊行为具有明显的季节性。在三月至六月期间采集样本的30天内，80％至100％的孤立囊肿被发现。然而，在8月份和2月初采集的样本中，只有0％和15％的样本存在。这种季节性特征在3年期间（2004-2007）反复出现。在从潮间潮汐位置和潮汐潮汐位置取样的囊肿中观察到这种效应。在塔玛亚历山大藻和A.的发芽中没有明显的内源时钟。从科克港采集的小型囊肿，在低氧条件下储存并保存在黑暗中长达18个月。观察到的季节效应与水温无关，虽然温度确实影响了放行率。温度也影响了实验室新形成的囊肿的成熟。还讨论了将季节性并入亚历山大藻的模型描述其增长的模型的意义。
AoifeNí Rathaille;RobinRaine;. Seasonality in the excystment of Alexandrium minutum and Alexandrium tamarense in Irish coastal waters[J]. Harmful Algae, 2011,10(6): 629-635