摘要
The aim of the present study was to determine potential role of leptin on in vitro developmental competence of buffalo oocytes and embryos. Slaughterhouse derived culture grade buffalo cumulus oocyte complexes (COCs) were matured in vitro (IVM) with leptin (10 ng/ml) or without leptin (control). In each experiment, a pool of matured COCs was used for further in vitro embryo production and another pool of COCs was used for cumulus cells and mature oocytes isolation to study the relative mRNA expression of developmentally important genes. Presumptive zygotes were cultured in embryo culture (IVC) media supplemented with leptin (10 ng/ml) or without leptin (control). Cleavage rate was higher (p < 0.05) when leptin was supplemented during IVM + IVC, both, as compared to other groups. Higher cleavage rate was observed in leptin-treated groups, though it was non-significant. Blastocyst rate was higher (p < 0.05) in all the leptin treated groups. The relative mRNA expression of LEPR (Ob-Rb), HAS2 and EGFR was significantly (p < 0.05) up-regulated and the expression of CASPASE3 was down-regulated in cumulus cells of leptin-treated groups. The expression of GDF9, BMP15, GLUT1, LEPR and CASPASE3 transcripts in leptin and non-treated oocytes did not differ. The relative mRNA expression of POU5F1and LEPR transcripts in blastocysts was higher (p < 0.05) in leptin-treated groups; the change in expression of GLUT1, INF-τ and CASPASE3 transcripts was not significant (p > 0.05). Thus, it is concluded that leptin promotes developmental competence of bubaline oocytes by modulating cumulus enabling factors and genes regulating pluripotentcy in the blastocysts.
摘要译文
本研究的目的是确定瘦素对水牛卵母细胞和胚胎体外发育能力的潜在作用。屠宰场衍生的文化级水牛卵母细胞复合物（COC）在体外成熟（IVM），瘦素（10ng / ml）或不含瘦素（对照）。在每个实验中，用于进一步的体外胚胎生产和另一个COC库用于卵丘细胞和成熟卵母细胞分离以研究发育重要基因的相对mRNA表达。推定受精卵在补充有瘦素（10ng / ml）或不含瘦素（对照）的胚胎培养（IVC）培养基中培养。切割率较高（p 0。05）当IVM IVC期间补充瘦素时，两者均与其他组相比。在瘦素治疗组中观察到较高的切割率，尽管它不显着。所有瘦素治疗组的囊胚率均较高（p 0.05）。 LEPR（Ob-Rb），HAS2和EGFR的相对mRNA表达显着（p 0。05）上调，瘦素治疗组卵丘细胞中CASPASE3的表达下调。 GDF9，BMP15，GLUT1，200新新新新旗新新新旗新新旗新新旗新新旗新新旗旗新200 200旗新新新旗新新旗新新旗新新200新新新旗新新200新新新旗新新200新新新200新新旗200新新新200新新新200新新新新旗新新200新新旗新新200新新新新旗新新200新新旗新新旗2001-新新新新新新200新新新200新新新200新新新旗200 X-45454545 X- 20045 X- 200 200 X- 20045 X- 200 200 X- 20045 X- 20045 X- 20045 X-05）在瘦素治疗组中; GLUT1，INF-τ和CASPASE3转录本的表达变化不显着（p 0.05）。从而，得出结论，瘦素通过调节囊泡使能因子和调节胚泡多能性的基因来促进泡沫卵母细胞的发育能力。
Bibhudatta S.K. Panda[a]; Sriti Pandey[a]; Anjali Somal[a]; Mehtab S. Parmar[a]; Irfan A. Bhat[a]; Indu Baiju[a]; Mukesh K. Bharti[a]; G. Sai Kumar[b]; Vikash Chandra[a]; G. Taru Sharma[a]. Leptin supplementation in vitro improved developmental competence of buffalo oocytes and embryos[J]. Theriogenology, 2017,98: 116–122