摘要
To investigate porcine Toll-like receptors (TLR) responding to viral pathogen associated molecular patterns, the full-length cDNA of porcine TLR3 and TLR7 were identified and characterized. Porcine TLR3 and TLR7 cDNA encode 904- and 1050-amnio-acid polypeptides, respectively. Both porcine TLR3 and TLR7 contain typical functional TLR domains and share about 80% sequence identity to other mammalian orthologues. Tissue expression profiles showed that TLR3 was highly expressed in kidney, duodenum, spleen and liver, and moderately expressed in bone marrow, lung, and skin. Conversely, TLR7 was moderately and constitutively expressed in all tissues evaluated. Stimulation of mammalian cells transfected with porcine TLR3 and TLR7 constructs with TLR3 and TLR7 agonists [poly (I:C) and imiquimod (R837), respectively], and adenovirus elicited activation of interferon regulatory factors (IRFs). These data provide molecular and functional information for porcine TLR3 and TLR7, and implicate their role in mediating immune protection against porcine viral diseases.
摘要译文
为了研究与病毒相关的分子模式相关的猪Toll样受体（TLR），鉴定和表征了猪TLR3和TLR7的全长cDNA。猪TLR3和TLR7 cDNA分别编码904和1050个羊膜酸多肽。猪TLR3和TLR7均含有典型的功能性TLR结构域，与其他哺乳动物直系同源物具有约80％的序列同一性。组织表达谱显示，TLR3在肾脏，十二指肠，脾脏和肝脏中高表达，在骨髓，肺和皮肤中表达中等。反过来，TLR7在所有评估的组织中适度和组成性表达。用TLR3和TLR7激动剂[聚（I：C）和咪喹莫特（R837）]分别刺激用猪TLR3和TLR7构建体转染的哺乳动物细胞，腺病毒引起干扰素调节因子（IRF）的激活。这些数据提供了猪TLR3和TLR7的分子和功能信息，并牵涉其在介导针对猪病毒疾病的免疫保护中的作用。
YongmingSang[a];JunYang[a];Chris R.Ross[a][1];Raymond R.R.Rowland[b];FrankBlecha[a];. Molecular identification and functional expression of porcine Toll-like receptor (TLR) 3 and TLR7[J]. Veterinary Immunology and Immunopathology, 2008,125(1-2): 162-167