摘要
CRISPR/Cas9-mediated gene editing may involve nonhomologous end-joining to create various insertion/deletions (indels) or may employ homologous recombination to modify precisely the target DNA sequence. Our understanding of these processes has been guided by earlier studies using other site-specific endonucleases, both in model organisms such as budding yeast and in mammalian cells. We briefly review what has been gleaned from such studies using the HO and I-SceI endonucleases and how these findings guide current gene editing strategies.
摘要译文
CRISPR / Cas9介导的基因编辑可能涉及非同源末端连接以产生各种插入/缺失（插入）或可能使用同源重组来精确修饰靶DNA序列。我们对这些过程的理解一直受到使用其他位点特异性核酸内切酶的早期研究的指导，包括模式生物体，如芽殖酵母和哺乳动物细胞。我们简要回顾一下使用HO和I-SceI核酸内切酶进行的研究以及这些发现如何指导当前的基因编辑策略。
Danielle N. Gallagher;James E. Haber[*]. Repair of a Site-Specific DNA Cleavage: Old-School Lessons for Cas9-Mediated Gene Editing[J]. ACS Chemical Biology, 2018,13(2): 397–405