摘要
Oxidative stress (OS) plays an important role in the process of ovarian granulosa cell apoptosis and follicular atresia. The aim of this study was to select antioxidant against OS in ovary tissue. Firstly, we chose the six antioxidants and analyzed the reactive oxygen species (ROS) level in the ovary tissue. The results showed that proanthocyanidins, gallic acid, curcumin, and carotene decrease the ROS level compared with control group. We further demonstrated that both proanthocyanidins and gallic acid increase the antioxidant enzymes activity. Moreover, change in the ROS level was not observed in proanthocyanidins and gallic acid group of brain, liver, spleen, and kidney tissues. Finally, we found that proanthocyanidins and gallic acid inhibit pro-apoptotic genes expression in granulosa cells. Taken together, proanthocyanidins and gallic acid may be the most acceptable and optimal antioxidants specifically against ovarian OS and also may be involved in the inhibition of granulosa cells apoptosis in mouse ovary.
摘要译文
氧化应激（OS）在卵巢颗粒细胞凋亡和滤泡闭锁过程中起着重要的作用。这项研究的目的是在卵巢组织中选择针对OS的抗氧化剂。首先选取6种抗氧化剂，分析卵巢组织中活性氧（ROS）水平。结果表明，原花青素，没食子酸，姜黄素，和胡萝卜素降低ROS水平与对照组相比。我们进一步表明，原花青素和没食子酸增加抗氧化酶活性。此外，在脑，肝，脾和肾组织中的原花青素和没食子酸组中没有观察到ROS水平的变化。最后，我们发现原花青素和没食子酸抑制颗粒细胞中促凋亡基因的表达。综合起来，蒽醌和没食子酸可能是卵巢OS特异性最好的抗氧化剂，也可能参与抑制卵巢颗粒细胞凋亡。
Bojiang Li[1]; Qiannan Weng[1]; Zequn Liu[1,2]; Ming Shen[1]; Jiaqing Zhang[1]; Wangjun Wu[1] and Honglin Liu[1,*];. Selection of antioxidants against ovarian oxidative stress in mouse model[J]. Journal of Biochemical and Molecular Toxicology, 2017,31(12)