摘要
Research up to 1993 is reviewed. Various methods for processing, freezing and thawing of semen have been elaborated and their effect on survival and fertility of spermatozoa have been examined in vitro or in fertility tests. Diluents initially used for freezing bull semen had their limitations and were modified or replaced by new media. The freezing diluents investigated included citrate-sugar-, milk-, lactose-, saccharose-, raffinose-, trisbased and other media containing protective agents (mainly glycerol and egg yolk), and other substances including antioxidants. Satisfactory sperm survival rates were obtained with a number of diluents and different combinations of the factors studied.
Lambing results after cervical insemination varied depending on the parameters examined in the freezing technology or at insemination, but were low in comparison with those obtainable with fresh diluted semen. As a consequence, cervical insemination with frozen-thawed semen had a relatively limited application in sheep.
摘要译文
研究到1993年进行审查。各种加工方法，已经详细阐述了精液的冷冻和融化，并且已经在体外或在生育力测试中检查了它们对精子的存活和繁殖的影响。最初用于冷冻公牛精液的稀释剂有其局限性，并被新媒体改变或取代。研究的冷冻稀释剂包括柠檬酸盐 - 糖 - ，乳 - ，乳糖 - 含有保护剂（主要是甘油和蛋黄）的蔗糖，棉子糖，三碱基和其它介质以及包括抗氧化剂在内的其他物质。用一些稀释剂和所研究因素的不同组合获得令人满意的精子存活率。宫颈授精后的结果根据在冷冻技术或受精中检查的参数而变化，但与用新鲜稀释精液获得的那些相比较低。因此，用冷冻精液进行宫颈授精在绵羊中应用相对有限。
S.Salamon;W.M.C.Maxwell;. Frozen storage of ram semen I. Processing, freezing, thawing and fertility after cervical insemination[J]. Animal Reproduction Science, 1995,37(3-4): 185-249