摘要
In this work three hundred and sixty strains of microorganisms were isolated from feces of nursing children and vaginal samples of ten women. Only twenty-two of the strains were identified as Lactobacillus. They were resistant to hostile conditions such as pH 3 and 0.30% of bile salts. It was detected antimicrobial activity in the growth supernatant against: Bacillus subtilis ATCC 60519, Candida albicans ATCC 14053, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 and against enteropatogenics bacteria strains such as Shigella sonnei, Escherichia coli poli I and Salmonella typhi isolated of infants with diarrhea. It was observed resistance of Lactobacillus strains to a mixture of 1.25 μg of trimethoprim and 23.75 μg of sulphamethoxazole. However they were sensitive to 10 μg of ampiciline. These strains can be considered as posibles probiotics of great utility in the dairy industry.
摘要译文
在这项工作中三百六十株微生物从护理孩子和十名女性阴道样本粪便中分离。只有22的菌株被鉴定为乳杆菌属。它们是耐敌对条件如pH值3和胆汁盐的0.30％。时，检测在对生长的上清液的抗微生物活性：枯草芽孢杆菌ATCC 60519，白色念珠菌ATCC 14053，大肠杆菌ATCC25922和金黄色葡萄球菌ATCC 25923和针对enteropatogenics菌菌株如志贺氏菌，大肠杆菌POLI I和伤寒沙门氏菌分离的婴儿腹泻。它被观察到乳杆菌菌株的抗性，以1.25微克的甲氧苄啶和23.75微克磺胺的混合物。然而，他们至10μgampiciline敏感。这些菌株可被认为是在乳品工业极大效用posibles益生菌。
MEJIA RODRIGUEZ, José Andrés, CHACON RUEDA, Zarack, GUERRERO CARDENAS, Balmore. Obtention of Lactobacillus strains. : In-Vitro Characterization as Potentials Probiotics.[J]. Revista Científica, 2007,17(2): 178-185