摘要
Based on our previous work, we found that exogenous oxytocin induces uterine tetany and cervical dilation, and permits transcervical access to the uterus. However, the oxytocin does not reduce sustained sperm transport from the uterus to the oviducts. Thus, we hypothesized that exogenous oxytocin may be a useful adjunct to transcervical intrauterine AI procedures for sheep: two experiments were conducted to test our hypothesis. In Experiment 1, purebred ewes (n = 75/group) were artificially inseminated intrauterine with either laparoscopic or oxytocin-transcervical (i.e., 200 USP units of oxytocin 30 min before AI) procedures. At 54 h after progestogenated pessaries were removed, ewes were inseminated with 200 × 106 sperm/0.25 ml of fresh, extended semen, which was collected from a purebred ram of the corresponding breed. Pregnancy rate was greater (P
摘要译文
基于我们以前的工作，我们发现，外源性催产素诱导子宫手足口病和宫颈扩张，并允许经子宫颈进入子宫。然而，催产素不会减少持续的精子从子宫输送到输卵管。因此，我们假设外源性催产素可能是一个有用的辅助子宫动脉子宫内膜异位症羊的程序：进行了两个实验来检验我们的假设。在实验1中，纯种的母羊（n \x3d 75 /组）是用腹腔镜或催产素 - 经宫颈（即AI前30分钟催产素为200UPS单位）人工授精的子宫内。孕激素避孕药后54小时，用200×10 6精子/ 0.25毫升新鲜的延长精液给母羊授精，该精液从相应品种的纯种公羊中收集。怀孕率更高（P
B.L.Sayre;G.S.Lewis;. Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes[J]. Theriogenology, 1997,48(2): 267-275