摘要
This study evaluated the synergistic efficiency of crude enzymatic extracts (CEE) produced by fungal strains Aspergillus fumigatus SCBM6 (AFE) and Aspergillus niger SCBM1 (ANE) in the hydrolysis of alkali pretreated sugarcane bagasse (2% NaOH (w v−1), ∼100 °C, 4 h and liquid-to-solid ratio of 25 (v w−1)). CEEs were produced by solid-state fermentation (SSF) and presented high concentration of β-glucosidase (AFE = 78.4 U g−1 and ANE = 43.02 U g−1), β-xylosidase (ANE = 78.0 U g−1) and xylanase (AFE = 1774.5 U g−1 and ANE = 3289.6 U g−1). A 24 full design of experiments was used to optimize the hydrolysis of pretreated bagasse as a function of time, temperature, amount of pretreated bagasse and AFE-to-ANE ratio. After hydrolysis optimization, the maximum concentration of total reducing sugars (TRS) was 21.68 g L−1. It was also noticed a significant production of xylose (135.59 mg) and arabinose (58.10 mg) per gram of hemicelluloses and a slight production of glucose (20.18 mg) per gram of cellulose. As fungi consortium were excellent producers of hemicellulases, it is suggested that they could be used for pentoses saccharification and production of ethanol via C-5 fermentation, xylitol, biopolymers, among other bioproducts.
摘要译文
在碱预处理的甘蔗渣（2％NaOH（w v -1））的水解中由真菌菌株烟曲霉SCBM6（AFE）和黑曲霉SCBM1（ANE）产生的马提克提取物（CEE）〜100℃，4小时，液固比为25（v w -1））。 CEEs通过固态发酵（SSF）生产，并呈现高浓度的β-葡萄糖苷酶（AFE \x3d 78。4 U g-1和ANE \x3d 43.02 U g -1），β-木糖苷酶（ANE \x3d 78.0 U g -1）和木聚糖酶（AFE \x3d 1774.5 U g -1和ANE \x3d 3289.6 U g -1）。使用2×4完全设计的实验来优化经预处理的甘蔗渣的水解作为时间，温度，经预处理的甘蔗渣的量和AFE与ANE之比的函数。水解优化后，总还原糖（TRS）的最大浓度为21.68 g L -1。还有人注意到木糖的一个显着的生产（135。59毫克）和阿拉伯糖（58.10毫克）/克半纤维素，每克纤维素产生少量葡萄糖（20.18毫克）。由于真菌协会是半纤维素酶的优秀生产者，建议它们可用于戊糖糖化和通过C-5发酵生产乙醇，木糖醇，生物聚合物等生物制品。
Patrísiade Oliveira Rodrigues[a];Josianide Cássia Pereira[b];Douglas Queirozdos Santos[c];Leandro Vinícius AlvesGurgel[d];DanielPasquini[e];Milla AlvesBaffi[a];. Synergistic action of an Aspergillus (hemi-)cellulolytic consortium on sugarcane bagasse saccharification[J]. Industrial Crops and Products, 2017,109: 173-181