摘要
Peripheral taste receptor cells use multiple signaling pathways to transduce taste stimuli into output signals that are sent to the brain. Transient receptor potential melastatin 5 (TRPM5), a sodium-selective TRP channel, functions as a common downstream component in sweet, bitter, and umami signaling pathways. In the absence of TRPM5, mice have a reduced, but not abolished, ability to detect stimuli, suggesting that a TRPM5-independent pathway also contributes to these signals. Here, we identify a critical role for the sodium-selective TRP channel TRPM4 in taste transduction. Using live cell imaging and behavioral studies in KO mice, we show that TRPM4 and TRPM5 are both involved in taste-evoked signaling. Loss of either channel significantly impairs taste, and loss of both channels completely abolishes the ability to detect bitter, sweet, or umami stimuli. Thus, both TRPM4 and TRPM5 are required for transduction of taste stimuli.
摘要译文
外周味觉受体细胞使用多种信号传导途径将味觉刺激转化为输送到大脑的输出信号。瞬时受体电位melastatin 5（TRPM5）是一种钠选择性TRP通道，可作为甜味，苦味和鲜味信号通路中常见的下游组分。在缺乏TRPM5的情况下，小鼠具有降低但未被废除的检测刺激的能力，表明TRPM5非依赖性途径也对这些信号有贡献。在这里，我们确定了钠选择性TRP通道TRPM4在味觉转导中的关键作用。在KO小鼠中使用活细胞成像和行为研究，我们显示TRPM4和TRPM5都参与味觉诱发信号。任一通道的损失都会显着损害品味，而两个通道的损失完全消除了检测苦味，甜味或鲜味刺激的能力。因此，TRPM4和TRPM5都是转导味觉刺激所必需的。
Debarghya Dutta Banik[1];Laura E. Martin[2];Marc Freichel[3];Ann-Marie Torregrossa[2];Kathryn F. Medler[1]. TRPM4 and TRPM5 are both required for normal signaling in taste receptor cells[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2018,115(4): E772-E781