摘要
The present study aimed to investigate the fertility of ewes artificially inseminated with three different methods using a synthetic semen extender, AndroMed. The three methods of artificial insemination (AI) were cervical AI with fresh-diluted or frozen-diluted semen at observed estrus, and an intrauterine AI with frozen-thawed semen. A total of 80 ewes were treated with a controlled internal drug release (CIDR) containing 0.3 g progesterone per device for 12 days. In Experiment 1 (26 Suffolk ewes), superovulation was induced with 20 mg follicle-stimulating hormone and 250 IU equine chorionic gonadotropin (eCG) two days and one day before CIDR removal, respectively, during the non-breeding season. In Experiment 2 (54 Suffolk and Suffolk crossbred ewes), an intramuscular injection of 500 IU eCG was administered one day before CIDR removal to synchronize estrus and ovulation during the breeding season. In Experiment 1, fresh-diluted or frozen-thawed semen was deposited into the cervical orifice after estrus detection, and an intrauterine AI with frozen-thawed semen was performed by laparoscopy at a fixed-time basis without estrus detection. Embryos were recovered by uterine flushing 6 days after AI, and the rates of recovered, fertilized (cleaved) ova and embryos at the morula or blastocyst stage were compared among the three AI methods. In Experiment 2, the pregnancy rates after the three AI methods were compared. In Experiment 1, the rates of recovered ova were not significantly different among the three AI methods (52.5-56.7%). The rate of fertilized ova (81.0%) by laparoscopic AI with frozen-thawed semen was significantly higher compared with cervical AI of fresh-diluted (25.5%) or frozen-thawed (3.5%) semen, but the rate of embryos at the morula or blastocyst stage (17.6%) was significantly lower than that of the cervical AI with fresh-diluted semen (69.2%). The rates of ewes yielding fertilized ova were not significantly different among the three groups (44.4, 11.1 and 62.5% for cervical AI with fresh-diluted and frozen-thawed semen and intrauterine AI with frozen-thawed semen). In Experiment 2, the pregnancy rate of ewes intrauterinally inseminated with frozen-thawed semen (72.2%) was significantly higher than those of ewes inseminated cervically with fresh-diluted (5.5%) or frozen-thawed (0.0%) semen. The present results showed that acceptable fertilization and pregnancy rates could be obtained by an intrauterine AI with frozen-thawed semen using a synthetic semen extender (AndroMed), but not sufficient by the cervical AI with either fresh or frozen semen.
摘要译文
本研究旨在探讨母羊人工授精与使用合成精液扩展，AndroMed三种不同方法的生育能力。人工授精（AI）的三种方法是颈椎AI新鲜稀释或冷冻精液稀释在发情观察，并宫内人工智能与冷冻精液解冻。共有80只母羊分别用含每台设备0.3克孕12天的内部控制药物释放（CIDR）。在实验1（26沙福母羊），超数排卵诱导用20毫克促卵泡激素和250 IU的马绒毛膜促性腺激素（ECG）两天1天CIDR去除之前，分别在非繁殖季节。在实验二（54萨福克郡和萨福克杂交母羊）注射500 IU心电图肌肉注射给药1天CIDR去除之前在繁殖季节同步发情和排卵。在实验1中，鲜稀释的或冷冻解冻的精液被存入宫颈口发情检测之后，和宫内人工智能与冷冻精液解冻通过腹腔镜在不发情检测固定时间的基础上进行。胚胎子宫冲洗恢复AI后第6天，和恢复，受精（裂）的卵子和胚胎的桑椹胚或囊胚期的利率分别在这三个AI方法相比。在实验2中，后三AI方法怀孕率进行了比较。在实验1，恢复卵率分别不三者间的AI方法（52.5-56.7％）显著不同。受精卵（81.0％）的腹腔镜AI率与冷冻精液解冻是显著高于新鲜稀释（25.5％）或冷冻解冻（3.5％）的精液颈椎AI相比，但胚胎在桑椹胚或囊胚期（17.6％）的速率比所述颈部的AI显著下用新鲜稀释精液（69.2％）。高产母羊卵子受精的发生率并没有三组（44.4，11.1和62之间显著不同。宫颈AI 5％新鲜稀释和冷冻精液解冻和宫内AI与冷冻精液解冻）。在实验2中，母羊人工授精intrauterinally用冷冻精液解冻（72.2％）的怀孕率明显高于母羊新鲜稀释（5受精与颈部显著高。5％）或冻融（0.0％）的精液。目前的结果显示，接受受精和怀孕率可能是由宫内人工智能与使用合成精液扩展冷冻精液解冻（AndroMed）获得，但不是由颈椎人工智能足够用新鲜或冷冻精液。
Mami HIWASA[1]; Hirohide KOHNO[2]; Tetsuro TOGARI[3]; Kentaro OKABE[2];Yutaka FUKUI[1]. Fertility after Different Artificial Insemination Methods Using a Synthetic Semen Extender in Sheep[J]. Journal of Reproduction and Development, 2009,55(1): 50-54