摘要
An experiment was undertaken to determine the conception rate of native sheep by using Artificial Insemination with liquid ram semen. The semen was collected from ram using artificial vagina and the was stored in a refrigerator (4°C) for three days. The volume of semen was extended with egg yolk citrate diluter. A total of 63 ewes were inseminated with stored liquid semen collected from 15 rams by AV method. The total number of spermatozoa, live-dead, normal-abnormal, sperm motility and the pH was observed regularly. The average semen volume per ejaculate was 0.76-1.00ml and the sperm concentration was 2.37x109 - 4.30x109 per ejaculate. The number of normal spermatozoa and the pH was almost similar irrespective of days of storage. Number of live spermatozoa and the sperm motility were reduced with the increasing age of semen. The average conception rate (%) was obtained as 63.61, 61.90, 52.38 and 47.61 with sperm in zero, 1st, 2nd and 3rd day storage respectively.
摘要译文
实验进行了利用人工授精液体公羊的精液来确定本地羊的受胎率。精液从公羊使用人工阴道收集并储存在冰箱中(4℃)三天。精液的体积扩展蛋黄柠檬酸稀释。共有63母羊人工授精与15公羊通过AV方法收集储存精液的液体。被经常观察到精子总数,活死,正常异常,精子活力和pH值。每次射精平均精液量为0.76-1.00ml和精子浓度是2.37x10 SUP 9 / SUP - 4.30x10 SUP 9 / SUP每次射精。正常的精子的数目和pH为存储的天几乎相似无关。活精子和精子活动数量减少精液的年龄增加。平均受孕率(%)于零,一,二,第3天,分别存放得到63.61,61.90,52.38和47.61精子。
S Pervage, MR Hassan, M Ershaduzzaman, MAMY Khandoker. Preservation of liquid semen and Artificial Insemination in native sheep[J]. Journal of the Bangladesh Agricultural University, 2009,7(2): 305-