摘要
Serum Ig from Trypanosoma congolense-infected cattle were affinity-purified using immobilised trypanosome or non-trypanosome antigens (ß-galactosidase, cytochrome C and ferritin). The bound and unbound IgG and IgM fractions were collected and tested in ELISA for reactivity to each antigen. The results indicated that the presence of reactivity to non-parasite antigens in serum of infected cattle is due to polyreactive IgM antibodies. However, the IgG fraction only bound to trypanosome antigens and was only present in post-infection sera, indicating that it was induced by the infecting trypanosomes. Since the polyreactive IgM antibodies were also present in pre-infection sera, it is probable that they were natural antibodies that were not induced but only amplified by the trypanosome infection.
摘要译文
使用固定的锥虫或非锥虫抗原（β-半乳糖苷酶，细胞色素C和铁蛋白）对来自Trypanosoma congolense感染的牛的血清Ig进行亲和纯化。收集结合和未结合的IgG和IgM级分并在ELISA中测试对每种抗原的反应性。结果表明，感染牛血清中对非寄生虫抗原的反应性是由于多反应性IgM抗体引起的。然而，IgG部分仅与锥体虫抗原结合，并且仅在感染后的血清中存在，表明它是由感染的锥虫体诱导的。由于多聚反应性IgM抗体也存在于感染前血清中，所以很可能它们是天然抗体，它们不是被诱导的，而是只被锥虫感染扩增。
JoramBuza[1];JanNaessens;. Trypanosome non-specific IgM antibodies detected in serum of Trypanosoma congolense-infected cattle are polyreactive[J]. Veterinary Immunology and Immunopathology, 1999,69(1): 1-9