摘要
The production of a recombinant baculovirus expression vector normally involves mixing infectious virus DNA with a plasmid-based transfer vector and then cotransfecting insect cells to initiate virus infection. The aim of this chapter is to provide an update on the range of baculovirus transfer vectors currently available. It is impractical to list every transfer vector that has ever been used. Instead, we focus on those that are available commercially and should be easy to locate. These vectors permit the insertion of single or multiple genes for expression, or the production of proteins with specific peptide tags that aid subsequent protein purification. A table listing the transfer vectors also included information on the parental virus that should be used with each one. Recent developments in recombinant baculovirus production are also described. Some of these permit the direct insertion of a recombinant gene into the virus genome without the requirement for a transfer vector. The information provided should enable new users of the system to choose those reagents most suitable for their purposes.
摘要译文
重组杆状病毒表达载体的制备通常包括将感染性病毒DNA与基于质粒的转移载体混合，然后共转染昆虫细胞以引发病毒感染。本章的目的是提供目前可用的杆状病毒转移载体范围的最新信息。列出曾经使用过的每个传输向量是不切实际的。代替，我们专注于那些商业上可用且易于定位的产品。这些载体允许插入单个或多个基因用于表达，或生产具有特定肽标签的蛋白质，以帮助随后的蛋白质纯化。列出转移载体的表还包括应与每个病毒一起使用的亲本病毒的信息。还描述了重组杆状病毒生产的最新发展。其中一些允许将重组基因直接插入病毒基因组而不需要转移载体。所提供的信息应该使系统的新用户能够选择最适合其目的的试剂。
Robert D. Possee[1];Linda A. King[2]. Baculovirus Transfer Vectors. Baculovirus and Insect Cell Expression Protocols[M].DE: Springer, 2007: 55-75