摘要
Baculoviruses are exceptionally attractive candidates as vectors for propagating and expressing exogenous DNAs in a eukaryotic (invertebrate) environment (Miller, 1981a). Among the features which make baculoviruses highly advantageous as recombinant DNA vector systems are (1) a covalently-closed, circular, nuclear-replicating DNA genome, (2) an extendable rod-shaped capsid, (3) a group of genes, involved in occlusion, that are nonessential for infectious virus production and thus deletable, and (4) a strong promoter which is turned on after infectious virus production and controls the synthesis of the major occlusion body protein (poly-hedrin), constituting approximately ten percent of the protein of infected cells. The replacement of the polyhedrin gene with passenger DNA was previously suggested as an approach to using baculoviruses as recombinant DNA vectors (Miller, 1981a). The initial experimental advances our laboratory has made in developing the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) as a vector in insect cells are described herein.
摘要译文
杆状病毒作为在真核（无脊椎动物）环境中繁殖和表达外源DNA的载体是特别有吸引力的候选物（Miller，1981a）。使杆状病毒作为重组DNA载体系统非常有利的特征之一是（1）共价闭合的环状核复制DNA基因组，（2）可延伸的杆状衣壳，（3）一组基因，参与闭塞，对于感染性病毒的产生是不必要的，因此是可删除的，和（4）在感染性病毒产生后开启并控制主要闭塞体蛋白（poly-hedrin）合成的强启动子，占大约百分之十的感染细胞的蛋白质。先前已经提出用载客DNA替换多角体蛋白基因作为使用杆状病毒作为重组DNA载体的方法（Miller，1981a）。本文描述了我们的实验室在开发杆状病毒苜蓿银纹夜蛾核多角体病毒（AcNPV）作为昆虫细胞中的载体方面所做的初步实验进展。
Lois K. Miller[1];David W. Miller[1];Michael J. Adang[1]. An Insect Virus for Genetic Engineering: Developing Baculovirus Polyhedrin Substitution Vectors. Genetic Engineering in Eukaryotes[M].DE: Springer, 1983: 89-97