摘要
The ability to make a large variety of virus-like particles (VLPs) has been successfully achieved in the baculovirus expression vector system (BEVS)/insect cell system. The production and scale-up of these particles, which are mostly sought as candidate vaccines, are currently being addressed. Furthermore, these VLPs are being investigated as delivery agents for use as therapeutics. Recently, adeno-associated viral (AAV) vectors, which can be potentially used for human gene therapy, have been produced in insect cells using three baculovirus vectors to supply the required genes. The use of host insect cells allows mass production of VLPs in a proven scaleable system. This chapter focuses on the methodology, based on the work done in our lab, for the production of AAV-like particles and vectors in a BEVS/insect cell system.
摘要译文
已经在杆状病毒表达载体系统（BEVS）/昆虫细胞系统中成功地实现了制备多种病毒样颗粒（VLP）的能力。目前正在解决这些颗粒的生产和扩大，这些颗粒主要作为候选疫苗。此外，正在研究这些VLP作为用作治疗剂的递送剂。最近，已经在昆虫细胞中使用三种杆状病毒载体产生可能用于人类基因治疗的腺相关病毒（AAV）载体以提供所需的基因。宿主昆虫细胞的使用允许在已证明的可扩展系统中大规模生产VLP。本章重点介绍基于我们实验室所做工作的方法，用于在BEVS /昆虫细胞系统中生产类似AAV的颗粒和载体。
Marc G. Aucoin[1];Danielle Jacob[1];Parminder Singh Chahal[1];Jamal Meghrous[1];Alice Bernier[1];Amine A. Kamen[1]. Virus-like Particle and Viral Vector Production Using the Baculovirus Expression Vector System/Insect Cell System. Baculovirus and Insect Cell Expression Protocols[M].DE: Springer, 2007: 281-296