摘要
In this paper the fundamental aspects of process development for the production of core and virus-like particles with baculovirus infected insect cells are reviewed. The issues addressed include: particle formation and monomer composition, chemical and physical conditions for optimal cell growth, baculovirus replication and product expression, multiplicity of infection strategy, and scale-up of the process. Study of the differences in the metabolic requirements of infected and non-infected cells is necessary for high cell density processes. In the bioreactor, the specific oxygen uptake rate (OURsp) plays a central role in process scale-up, leading to the specification of the bioreactor operational parameters. Shear stress can also be an important variable for bioreactor operation due to its influence on cell growth and product expression.
摘要译文
本文的工艺开发用于生产芯和病毒样颗粒的与感染杆状病毒的昆虫细胞的基本方面进行了综述。所解决的问题包括：颗粒形成和单体组合物，化学和最佳细胞生长，杆状病毒复制和产物表达的物理条件，感染策略的多样性，和放大的过程。研究在感染和非感染细胞的代谢需求的差异是必要的高细胞密度的过程。在生物反应器中，特定的氧吸收速率（ OURsp ）起着工艺放大中心作用，导致该生物反应器的操作参数的指定。剪切应力也可以是生物反应器的操作的一个重要变量，由于其对细胞生长和产物表达的影响。
Luis Maranga (20) Pedro E. Cruz (20) (21) John G. Aunins (20) (22) Manuel J. T. Carrondo (20) (23). Production of Core and Virus-Like Particles with Baculovirus Infected Insect Cells. Tools and Applications of Biochemical Engineering Science[M].DE: springer, 2002: 183-206