图书章节

Simultaneous Production of Xylanase and Mannanase by Several Hemicellulolytic Fungi 收藏

几种半纤维素分离菌同时生产木聚糖酶和甘露聚糖酶
原文求助 发布源
作者
G. M. Gübitz and Walter Steiner
作者单位
Enzyme Technology Laboratory, Institute of Biotechnology, Technical University Graz, Petersgasse 12, A-8010 Graz, Austria
发布日期
May 05, 1996
页码
319-331
DOI
10.1021/bk-1995-0618.ch020
来源信息
Enzymatic Degradation of Insoluble Carbohydrates ISBN：9780841233416, 1996年, 319-331页
摘要
The simultaneous production of xylanase and mannanase by the hemicellulolytic fungi Thermomyces lanuginosus, Sclerotium rolfsii and Schizophyllum commune was studied. Substrate-optimization experiments were carried out by using statistical design methods to obtain a maximal mannanase activity under the constraint of a certain high level of xylanase activity. To increase the level of mannanase activity different mannan-rich carbon sources such as konjak powder, locust bean gum, copra meal powder and others, were added to the culture media formerly optimized for xylanase production. The highest level of mannanase activity (3,3 μkat/ml) was detected in the culture filtrate of S. rolfsii when grown on a mixture of α-cellulose and konjak powder as carbon source. The secreted mannanases were characterized by their molecular weights, pI values and pH and temperature optima and stabilities.
摘要译文
通过半纤维素分解真菌Thermomyces lanuginosus，Sclerotium rolfsii和Schizophyllum commune同时产生木聚糖酶和甘露聚糖酶。通过使用统计设计方法进行底物优化实验，以在一定高水平的木聚糖酶活性的约束下获得最大甘露聚糖酶活性。为了提高甘露聚糖酶活性水平，将不同富含甘露聚糖的碳源如魔芋粉，刺槐豆胶，椰子粕粉等添加到先前针对木聚糖酶生产优化的培养基中。当在作为碳源的α-纤维素和魔芋粉末的混合物上生长时，在S. rolfsii的培养滤液中检测到最高水平的甘露聚糖酶活性（3,3μkat/ ml）。分泌的甘露聚糖酶的特征在于它们的分子量，pI值和pH和温度最佳值和稳定性。
G. M. Gübitz and Walter Steiner. Simultaneous Production of Xylanase and Mannanase by Several Hemicellulolytic Fungi. Enzymatic Degradation of Insoluble Carbohydrates[M].US: ACS, 1996: 319-331