摘要
Estrogen loss-induced bone remodeling and microdamage accumulation may play significant roles in postmenopausal bone fragility. We asked whether the adult female ferret (Mustela putorius furo) is suitable as a small animal model in which to study estrogen loss-induced bone remodeling, and whether the rat ulnar end-load bending model could be adapted to the ferret ulna for the study of microdamage-induced remodeling in cortical bone. To determine whether ovariectomy results in a significant increase in cortical and trabecular bone remodeling in adult female ferret, we ovariectomized 8 female ferrets, while an additional 8 ferrets served as intact controls. All animals were given in vivo bone labels 10 and 3 days pre-euthanasia and were sacrificed 45 days post-ovariectomy. Histomorphometric analysis was performed of the ulna, femoral neck (FN), and secondary lumbar vertebral body (L2), and ulnae were examined for microdamage. To adapt the ulnar end-load bending model for the ferret, we determined the ex vivo mechanical properties of the ferret ulna-radius complex, the ex vivo load-strain relationship in the ulna and radius, and the relative strain magnitudes in the ulna and radius, all of which are required preliminary steps in the development of an in vivo loading protocol. We found that resorption cavity density (rCv.Dn) was 290% greater, activation frequency (Ac.f) was 470% greater, and bone formation rate (BFR) was 880% greater in ovariectomized than intact, estrous ferret ulnar cortical bone. Ovariectomy did not affect trabecular bone remodeling in the FN or L2. The ulna did not contain any visible microdamage. The maximum load of the intact ulna-radius complex during monotonic loading to failure was 55 N. The load-strain relationship for the ulna and radius were approximately quadratic. Strain was approximately 1 to 8 times greater in the ulna than in the radius depending on the load; as load increased, the ulna experienced more strain relative to the radius. Our data suggest that the female ferret may be useful for the study of estrogen loss-induced cortical bone remodeling and that the end-load bending model may be adapted to the ferret ulna.
摘要译文
雌激素缺失诱导的骨重建和微损伤累积可能在绝经后骨脆性中起重要作用。我们询问成年雌鼬（Mustela putorius furo）是否适合作为研究雌激素损失引起的骨重建的小动物模型，以及大鼠尺骨末端负重弯曲模型是否适用于白鼬尺骨，以研究皮质骨微毁损重建。为了确定卵巢切除术是否导致成年女性雪貂的皮质和骨小梁骨重建显着增加，我们去卵巢8女性雪貂，另有8只雪貂作为完整的控制。所有动物在安乐死之前10天和3天被给予体内骨标记，并在卵巢切除术后45天处死。对尺骨，股骨颈（FN）和继发性腰椎椎体（L2）进行组织形态计量学分析，并检查尺神经损伤。为了适应雪貂的尺骨末端弯曲模型，我们确定了雪貂尺桡骨复合体的离体力学性能，尺骨和桡骨的体外负荷 - 应变关系以及尺骨和桡骨的相对应变量，[所有这些都是开发体内负载方案所需的初步步骤。我们发现吸收腔密度（rCv.Dn）增加了290％，活化频率（Ac。）f）比正常发情的白鼬皮质骨高470％，骨形成率（BFR）高出880％。卵巢切除术不影响FN或L2的小梁骨重塑。尺骨不包含任何可见的微损伤。完整尺骨半径复合体在单调加载失败时的最大载荷为55N。尺骨和半径的载荷 - 应变关系近似二次方。尺寸上的应变大约是根据负荷的半径的1至8倍;当负荷增加时，尺骨相对于半径经历更多的应变。我们的数据表明，女性雪貂可能是有用的研究雌激素损失引起的皮质骨重塑和终端负载弯曲模型可能适应白鼬尺骨。
Castillo, Alesha Bernedette. The female ferret (Mustela putorius furo) as an in vivo model for skeletal research[D]. US: University of California, Davis, 2004