博硕论文

Isolation and purification of angiogenesis inhibitor from Flemingia macrophylla 收藏

来自Flemingia macrophylla的血管生成抑制剂的分离和纯化

原文求助

学校

California State University, Fresno(加利福尼亚州立大学弗雷斯诺分校)

作者

Qutob, Samer Samir

关键词

Health and environmental sciences; Pure sciences

关键词译文

健康与环境科学;纯科学

来源信息

California State University, Fresno，学位：M.S.，导师：Wong, Kin-Ping, 2004年, 44页

摘要

Flemingia macrophylla, a plant native to Southeast Asia, was investigated to isolate an angiogenesis inhibitor from its roots. An aqueous extract from the roots proved to exert a prominent inhibitory effect on bovine cardiopulmonary artery endothelial cells (CPAE) in endothelial cell culture assay. The inhibitory effect was concentration dependent. The different parts of the root were investigated for the presence of the angiogenesis inhibitor. Extracts from both the periderm (bark) and the vascular tissue (wood) of the root inhibited the Bovine (CPAE) cells, but the periderm had the highest concentration of the inhibitor compared to the other tissue of the root. The root extract proved to have an activity against bovine (CPAE) but had no activity against human prostate cancer cells (LNCaP). High Performance Liquid Chromatography was used to isolate the fraction of the root extract that exerted the inhibitory activity. Subsequent trials were done to study the characteristics of the inhibitor using the carbohydrate and protein assays, U.V.-visible spectrophotometry and mass spectrometry.

摘要译文

调查了东南亚当地植物弗莱明亚大麦（Flemingia macrophylla），从其根部分离血管生成抑制剂。来自根的水提取物证明在内皮细胞培养测定中对牛心肺动脉内皮细胞（CPAE）具有显着的抑制作用。抑制作用是浓度依赖性的。研究了根部的不同部位的血管生成抑制剂的存在。来自根的皮质（树皮）和血管组织（木材）的提取物抑制牛（CPAE）细胞，但与根部的其他组织相比，皮质与抑制剂的浓度最高。根提取物证明具有针对牛（CPAE）的活性，但对人前列腺癌细胞（LNCaP）没有活性。高效液相色谱法用于分离提取抑制活性的根提取物的分数。进行随后的试验以使用碳水化合物和蛋白质测定法，U.V.-可见分光光度法和质谱法来研究抑制剂的特征。

Qutob, Samer Samir. Isolation and purification of angiogenesis inhibitor from Flemingia macrophylla[D]. US: California State University, Fresno, 2004