摘要
In this study, we examined the levels of leptin and OB-Rb protein expression in the discrete areas of the porcine hypothalamus (mediobasal hypothalamus – MBH, pre-optic area – POA, stalk median eminence – SME) during mid- and late-luteal phases of the oestrous cycle (days 10–12 and 14–16) as well as two stages of pregnancy (days 14–16 and 30–32). The analysis showed that during the cycle, leptin protein expression in MBH was higher in the mid-luteal phase than late-luteal phase. In the case of OB-Rb protein expression, a higher level was observed in MBH during the late-luteal phase in comparison to the mid-luteal phase, whereas in POA and SME the opposite dependence was noticed. In turn, during pregnancy, leptin protein expression in MBH and POA, and OB-Rb protein expression in POA were more pronounced on days 14–16 than on days 30–32. In contrast, leptin protein content in SME as well as OB-Rb protein in MBH and SME was higher on days 30–32 than during the earlier stage of pregnancy. Comparison of leptin and OB-Rb protein expression between the cycle (days 10–12) and pregnancy showed a higher level of leptin and OB-Rb protein contents in POA as well as in SME during pregnancy (on days 14–16 and 30–32, respectively). Yet, OB-Rb protein expression in POA on days 30–32 of pregnancy was lower in comparison to days 10–12 of the cycle. Furthermore, during pregnancy, leptin protein expression in MBH was lower (days 14–16 and 30–32), whereas OB-Rb protein expression in that area of hypothalamus was higher (days 30–32) in comparison to the mid-luteal phase. Our results indicate that both leptin and OB-Rb are synthesized in the porcine hypothalamus and suggest the participation of leptin in auto/paracrine regulation of these brain areas functions, including control of reproduction during the oestrous cycle and early pregnancy.
摘要译文
在这项研究中，我们检查了瘦素和OB-Rb蛋白的表达水平在猪丘脑的离散区域（mediobasal丘脑 - MBH，预光学区域 - POA，秆正中隆起 - SME）在发情周期的中期和后期黄体期（10-12天及14-16），以及两个阶段怀孕（天14-16和30-32）。分析结果表明，在循环过程中，在MBH瘦素蛋白表达更高中期黄体期比晚黄体期。在OB-Rb蛋白表达的情况下，更高水平中观察到MBH在晚期黄体期相比，黄体中期阶段，而在POA和中小企业相反依赖性被发现。反过来，在怀孕期间，在MBH和POA，并在POA OB-Rb蛋白表达瘦素蛋白的表达水平在14-16天比30-32天更明显。相反，中小企业瘦素蛋白含量以及在MBH和中小企业OB-Rb蛋白是在30-32天比在怀孕前期较高。和周期（天10-12）和妊娠之间的OB-Rb蛋白表达表明瘦素和POA以及中小企业孕期OB-Rb蛋白含量（按天14-16和30-32的一个更高的水平，分别）。然而，在天中的POA OB-Rb蛋白表达妊娠30-32较低相比天周期的10-12。此外，在怀孕期间，在MBH瘦素蛋白表达量较低（14-16天30-32和），而下丘脑的面积OB-Rb蛋白表达量较高（30-32天），相较于黄体中期阶段。我们的研究结果表明，这两个瘦素和OB-Rb在猪丘脑合成并显示瘦素在这些脑区的功能自动/旁分泌调节的参与，包括复制的发情周期和早孕期控制。
G Siawrys, T Kaminski, N Smolinska and J Przala. Expression of Leptin and Long-form Leptin-receptor Proteins in Porcine Hypothalamus during Oestrous Cycle and Pregnancy[J]. Reproduction in Domestic Animals, 2009,44(6): 920-926