摘要
Aims: The aim of the present study was to monitor the changes in the composition of microbiota in solid-state fermented feed and to evaluate their biosafety.
Methods and Results: In the solid-state fermentation, six probiotic bacteria strains were used as inoculum and soybean meal were used as carbohydrate source. At 0, 1, 2, 3, 5, 7, 10 and 15 days, samples were collected for further analysis. Denaturing gradient gel electrophoresis (DGGE) analysis showed that Bifidobacterium bifidum and Bacillus licheniformis were always present throughout the fermentation. Bifidobacterium bifidum, Enterococcus faecalis and Lactobacillus acidophilum were dominant throughout the entire fermentation as monitored by Lactobacillus-specific PCR–DGGE. Probiotics supplementation could reduce the levels of the pathogenic bacteria such as Staphylococcus aureus and enterotoxigenic Escherichia coli (ETEC) by species-specific real-time PCR. And Salmonella spp. was not detected throughout the entire fermentation.
Conclusions: Probiotics supplemented are always dominant throughout the whole period of solid-state fermentation and effective in preventing the growth of pathogens.
Significance and Impact of the Study: Based on the results, the high quality, stable solid-state fermented feed could be produced and applied in the pigs to improve the animal performances.
摘要译文
目的：本研究的目的是监测该变化菌群在固态发酵饲料用组合物，并评价其生物安全。方法和结果：在固态发酵，六益生菌菌株被用作接种物和大豆粉被用作糖源。在0，1，2，3，5，7，10和15天，收集用于进一步分析的样品。变性梯度凝胶电泳（DGGE）分析表明，双歧杆菌和地衣芽孢杆菌总是整个发酵本。双歧杆菌，粪肠球菌和乳嗜酸是整个发酵占主导地位的乳酸杆菌特异性PCR-DGGE的监控。益生菌补充剂可以减少致病细菌如金黄色葡萄球菌和肠毒素大肠杆菌（ETEC）通过物种特异性实时PCR的水平。和沙门氏菌。在整个发酵中没有检测到。结论：益生菌补充总是主导整个固态发酵的整个周期和有效地防止病原体的生长。意义和研究的影响：根据调查结果，高品质，稳定的固态发酵饲料能生产和猪的应用提高了动物表演。
Z. Yu, B. Dong and W. Lu. Dynamics of bacterial community in solid-state fermented feed revealed by 16S rRNA[J]. Letters in Applied Microbiology, 2009,49(2): 166-172