摘要
Non-steroidal anti-inflammatory drugs (NSAIDs) are classified as Class 4 agents by the Association of Racing Commissioners International and are banned in racehorses during competition in Pennsylvania (PA). To control the abuse of these agents in racehorses competing in PA, a forensic method for screening and confirmation of the presence of these agents is needed. Equine plasma (0.5 mL) was acidified with 75 μL 1M H[3]PO[4] to increase recovery of the analytes by liquid-liquid extraction using methyl tert-butyl ether (MTBE). Extracted analytes were separated by reversed-phase liquid chromatography using a C[8] column under gradient condition. All 16 analytes were detected, quantified and confirmed using a triple quadrupole tandem mass spectrometry with selected reaction monitoring (SRM) in both negative and positive electrospray ionization modes. The limit of detection, quantification and confirmation of the analytes were 1.0 - 5.0 ng/mL, 1.0 - 5.0 ng/mL and 1.0 - 20 ng/mL, respectively. The linear dynamic range of quantification was 5.0 - 200 ng/mL. The method is routinely used in anti-doping analysis to control the abuse of NSAIDs in racehorses competing in PA.
摘要译文
非甾体抗炎药（NSAIDs）是由赛车专员国际协会列为4级代理和宾夕法尼亚州（PA）在比赛中是被禁止的赛马。为了控制这些药剂在赛马中的PA竞争的滥用，需要一种用于筛选和确认这些试剂的存在的法医方法。马血浆（0.5毫升）酸化用75μL1M H [3] PO [4]，以增加通过液 - 液萃取用甲基叔丁基醚（MTBE）回收所述分析物。提取分析物通过使用梯度条件下为C [8]柱反相液相色谱法分离。进行检测，量化并确认使用三重四极杆串联质谱选择反应监测（SRM）的负极和正极电喷雾电离模式下的所有16分析。的检测，定量和确认分析物的极限为1.0 - 5.0纳克/毫升，1.0 - 5.0 ng / mL和1.0 - 20毫微克/毫升，分别。量化的线性动态范围为5.0 - 200毫微克/毫升。该方法是经常用在反兴奋剂的分析来控制的NSAIDs的赛马在PA竞争的滥用。
Youwen You1*; Cornelius E. Uboh2; Fuyu Guan1; Lawrence R. Soma1
. Doping Control Analysis of 16 Non-Steroidal Anti-Inflammatory Drugs in Equine Plasma Using Liquid Chromatography-Tandem Mass Spectrometry[J]. American journal of analytical chemistry, 2014,5(17)