期刊文献

Virus-like particles of hepatitis B virus core protein containing five mimotopes of infectious bursal disease virus (IBDV) protect chickens against IBDV 收藏

含有感染性囊病病毒的5模拟表位（IBDV）防止IBDV鸡乙型肝炎病毒核心蛋白的病毒样颗粒

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作者

Yong-shan Wang[a]; Wei Ouyang[a]; Xiao-juan Liu[a][b]; Kong-wang He[a]; Sheng-qing Yu[c]; Hai-bin Zhang[b]; Hong-jie Fan[b]; Cheng-ping Lu[b]

作者单位

[a] Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China [b] Department of Microbiology and Immunology, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China [c] Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China

关键词

IBDV, infectious bursal disease virus; 5EPIS, a polypeptide consisting of 5 epitopes, actually mimotopes, of IBDV VP2 protein linked in tandem with short amino acid spacers; HBc, hepatitis B core protein; VLP, virus-like particles

关键词译文

IBDV，鸡传染性法氏囊病病毒; 5EPIS，多肽，由5表位，模拟表位实际上IBDV VP2蛋白中配合短氨基酸间隔物相连的;的HBc，乙型肝炎核心蛋白; VLP，病毒样颗粒

发布日期

26 January 2012

页码

2125–2130

DOI

10.1016/j.vaccine.2012.01.040

来源信息

Vaccine ISSN：0264-410X, 2012年, 30卷, 12期, 2125–2130页

摘要

Current infectious bursal disease virus (IBDV) vaccines suffer from maternal antibody interference and mimotope vaccines might be an alternative. Previously we demonstrated an IBDV VP2 five-mimotope polypeptide, 5EPIS, elicited protective immunity in chickens. In the current study, the 5epis gene was inserted into a plasmid carrying human hepatitis B virus core protein (HBc) gene at its major immunodominant region site. The recombinant gene was efficiently expressed in Escherichia coli to produce chimeric protein HBc-5EPIS which self-assembles to virus-like particles (VLP). Two-week old specific-pathogen-free chickens were immunized intramuscularly with HBc-5EPIS VLP or 5EPIS polypeptide without adjuvant (50 μg/injection) on day 0, 7, 14 and 21. Anti-5EPIS antibody was first detected on day 7 and day 21 in HBc-5EPIS and 5EPIS groups, respectively; on day 28, anti-5EPIS titers reached 12,800 or 1600 by ELISA, and 3200 or 800 by virus neutralization assay in HBc-5EPIS and 5EPIS groups, respectively. No anti-5EPIS antibody was detected in the buffer control group throughout the experiment. Challenge on day 28 with a virulent IBDV strain (GX8/99) resulted in 100%, 40.0% and 26.7% survival for chickens immunized with HBc-5EPIS, 5EPIS and buffer, respectively. These data suggest epitope presentation on chimeric VLP is a promising approach for improving mimotope vaccines for IBDV.

摘要译文

当前传染性法氏囊病病毒（IBDV）的疫苗从母体抗体干扰遭受和模拟表位疫苗可能是一种选择。以前我们展示了一个IBDV VP2五模拟表位肽，5EPIS，引起鸡群的免疫保护。在目前的研究中，该5epis基因插入一个质粒携带人乙型肝炎病毒核心蛋白（HBC）基因在其主要的免疫显性区部位。该重组基因高效表达在大肠杆菌中以产生嵌合蛋白的HBc-5EPIS其中自组装到病毒样颗粒（VLP）。二周龄特定无病原体的鸡用的HBc-5EPIS VLP或5EPIS多肽不含佐剂（50肌内免疫首先对的HBc-5EPIS和5EPIS团，分别为7天和21天检测到的抗5EPIS抗体;在第28天，抗5EPIS滴度达到12800或1600通过ELISA，与3200或800，分别病毒中和试验中HBC-5EPIS和5EPIS组。在整个实验过程中的缓冲器控制组中没有检测到抗5EPIS抗体。挑战第28天用有毒IBDV毒株（GX8 / 99）导致100％，40.0％和26.7％，存活的鸡免疫HBC-5EPIS，5EPIS和缓存，分别为。这些数据表明在嵌合VLP抗原展示是一种很有前途的方法来提高模拟表位疫苗毒株。

Yong-shan Wang[a]; Wei Ouyang[a]; Xiao-juan Liu[a][b]; Kong-wang He[a]; Sheng-qing Yu[c]; Hai-bin Zhang[b]; Hong-jie Fan[b]; Cheng-ping Lu[b]. Virus-like particles of hepatitis B virus core protein containing five mimotopes of infectious bursal disease virus (IBDV) protect chickens against IBDV[J]. Vaccine, 2012,30(12): 2125–2130