摘要
The aim of this study was to compare the efficiency of the intravaginal (IVAI) vs. intrauterine artificial insemination (IUAI) using frozen–thawed sperm in the domestic cat. Semen was collected from two tom cats using an artificial vagina and samples were assessed for motility (computer-assisted sperm analysis (CASA)), sperm morphology and plasma membrane integrity. After dilution with TRIS/OEP/YOLK (4% of glycerol), sperm samples were loaded into 0.25 mL straws (25 × 106 motile sperm/straw), incubated at 5 °C for 20 min and cryopreserved over liquid nitrogen (LN2) vapor for 15 min and then immersed in LN2. For each AI, four straws from the same male were thawed (12 s at 46 °C) and centrifuged at 250 × g for 8 min to pellet the sperm. The supernatant was discarded and sperm pellet resuspended with the remaining liquid, approximately 100 μL, and analyzed as described above. Queens were treated with a single im injection of 100 IU eCG to induce ovarian follicular development. Final oocyte maturation and ovulation was induced with 100 IU hCG given im at 82–84 h after eCG administration. Thirty hours after hCG administration, females were inseminated either intrauterine (n = 8 queens) or intravaginally (n = 8 queens), using thawed sperm from a single male. Although a pronounced decrease in sperm motility, acrosome and plasma membrane integrity was observed in sperm samples from both cats, a pregnancy rate of 75% was achieved when using the intrauterine AI method compared with 0% pregnancy when inseminated intravaginally.
摘要译文
这项研究的目的是比较使用冷冻精液解冻后，在国内猫阴道（IVAI）与宫腔内人工授精（IUAI）的效率。精液从两个汤姆猫使用人工阴道采集和样品被评定为动力（计算机辅助精子分析（CASA）），精子形态和细胞膜的完整性。稀释含TRIS / OEP /蛋黄（甘油的4％）后，精子样品装入0.25毫升吸管（25×10 SUP 6 / SUP活动精子/秸秆），在5℃下进行20分钟，并冷冻保存在液氮（LN 2）的蒸气进行15分钟，然后浸渍于LN 2。对于每一个AI，来自相同雄性4吸管分别（在46℃12秒）解冻并在250×g下离心8分钟以沉淀的精子。丢弃上清液和精子沉淀再悬浮与剩余的液体，约100皇后区均采用单一肌肉注射100 IU心电图诱发卵巢卵泡发育。最后的卵母细胞成熟和排卵诱导用100 IU的hCG IM给予心电监护，在给药后82〜84小时。三十个小时的hCG施用后，雌性受精或者宫内（N \x3d 8皇后），或阴道内（N \x3d 8皇后），使用解冻的精子从单一的男性。虽然精子活力显下降，顶体和细胞膜完整性的观察精子样本中的两个猫，75％的怀孕率，用0％怀孕阴道内授精相比宫内人工智能方法时实现的。
Ana Izabel Silva Balbin Villaverde[a]; Cely Marini Melo[a]; Ian Martin[a]; Tatiana Henriques Ferreira[b]; Frederico Ozanam Papa[a]; Cesar Augusto Taconeli[c]; Maria Denise Lopes[a]. Comparison of efficiency between two artificial insemination methods using frozen–thawed semen in domestic cat (Felis catus): Artificial insemination in domestic cats[J]. Animal Reproduction Science, 2009,114(4): 434–442