摘要
Introduction There is a great need for better understanding of the intestinal bioavailability of B-vitamins and the factors that influence it. In food products, however, nutrient content does not necessarily imply the proportion of nutrient content actually absorbed. Thus a variety of experimental approaches have been used for determining the intestinal bioavailability of food vitamins. They include measuring changes in tissue vitamin levels, urinary vitamin excretion and growth in response to known intakes of vitamin-containing foods (e.g. Southern and Baker 1981; Gregory and Litherland 1986; Abad and Gregory 1987; Clifford et al. 1990; Swatilo et al. 1990; Yu and Kies 1993; Matte and Girard 1994). Potential limitations of those animal bioassays centre mainly on the role of the intestinal microflora and the extent of enhancement or inhibition of their vitamin synthesis by dietary components. The endogenous vitamin synthesis by the intestinal flora, with possible uptake in the distal part of the small and/or large intestine, is a complicating factor for nearly all B-vitamins. The components of test diets that would stimulate the synthesis of B-vitamins by intestinal microorganisms could therefore cause overestimation of dietary vitamin availability in conventional bioassays. Application of in-vitro assays has also been reported for water-soluble vitamins, but these are not recommended because of the limitations in extrapolation of the results to the situation in vivo (Holler et al. 1975; Seyoum and Selhub 1998). In principle, methods using stable isotopes will give the most reliable information concerning intestinal absorption, but their application is still limited, mainly because stable isotopes are not commercially available and specialized facilities and expertise are required. Ileo-rectal anastomosis has been proposed as an alternative method for calculating the intestinal availability of nutrients (Herrmann et al. 1988; Green and Kiener 1989; Laplace et al. 1989; Hennig et al. 1990; Roth-Maier et al. 1998). The most important advantage of ileo-rectal anastomosis for measuring vitamin digestibility is the prevention of vitamin synthesis by the intestinal microflora and coprophagy, because the digesta can be collected quantitatively via the anus. In this respect two different techniques of ileo-rectal anastomosis have been developed for pigs: the end-to-side (ESV) and end-to-end (EEV) ileo-rectal anastomosis with preserved ileo-caeco-colic valve. Both methods have already been compared for their use as digestibility assays for the vitamins including thiamin, riboflavin and vitamin B6 (Roth-Maier et al. 1998). The objective of the present study was to compare both the ESV and EEV techniques with regard to the digestibility values of folate, niacin and pantothenic acid, which has not been tested hitherto. In addition, in order to examine age- or time-related effects on vitamin digestibility two different experimental periods after surgery were chosen for measurement. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } Get PDF (217K)Get PDF (217K) More content like thisFind more content: like this articleFind more content written by: A. Wauer G. I. Stangl M. Kirchgessner W. Erhardt J. Henke U. Hennig D. A. Roth-Maier All Authors
摘要译文
简介有是为更好地了解B族维生素肠道生物利用度和影响ITIN食品的因素有很大的需求，但是，养分含量并不一定意味着营养素含量实际吸收的比例因此各种实验方法已被用于确定食品的肠生物利用度vitaminsThey包括在组织中维生素水平测量的变化，尿排泄的维生素和增长响应的含维生素的食物（E竒南部和贝克1981年著名的摄入量; Gregory和Litherland 1986;阿巴德和格雷戈里1987年，克利福德等al1990;Swatilo等al1990;羽和1993年Kies的;磨砂和1994年吉拉德）这些动物的生物测定中心的潜在的局限性主要是对肠道菌群的作用，并增强或它们的维生素合成的抑制的通过膳食成分的程度内源性维生素合成通过肠道菌群，与在小和/或大肠的末端部分可能摄取，是一个复杂的因素几乎所有B族维生素因此，他将刺激肠道微生物B族维生素的合成试验日粮成分可能会导致传统的生物测定的饮食中维生素可用性高估也已报道了水溶性维生素的体外测定法中的应用，但这些不推荐，因为在结果的情况在体内外推的局限性（奥莱等al1975;塞尤姆和1998年Selhub）原则上，使用稳定同位素方法会给关于肠道吸收的最可靠的信息，但它们的应用仍然有限，这主要是因为稳定同位素不是市售和专业设施和专业知识是必需回肠 - 直肠吻合术已经被提出作为一种替代方法，用于计算营养素的肠可用性（赫尔曼等al1988; Green和KIENER 1989;拉普拉斯等al1989;亨尼格等al1990;罗斯-Maier等al1998）用于测量维生素消化率回肠直肠吻合术的最重要的优点是防止维生素合成通过肠道菌群和食粪，因为食糜能够定量经肛门被收集这方面回肠直肠吻合术的两种不同的技术已被用于猪开发：端至端（ESV）和端 - 端（EEV）回肠 - 直肠吻合保存回肠caeco绞痛阀这两种方法已经为自己的消化实验的维生素包括硫胺素，核黄素和维生素B 6使用比较（罗斯-Maier等al1998）本研究的目的是对于叶酸，烟酸和泛酸的消化率，而没有被迄今测试，以比较这两个ESV和EEV技术此外，为了检查对维生素消化率年龄或时间相关的影响手术后两个不同的实验期间被选为测量上划线{文字修饰：上划线; }竤卡车{文字装饰：线通过; }竨nderlined {文本装饰：下划线; }竏oubleUnderlined {文本装饰：下划线;下边框：1px的固体＃000;}获取PDF（217K）获取PDF（217K）更多内容，如thisFind更多的内容：AWauer GIStangl MKirchgessner WErhardt JHenke UHennig DA：像这个articleFind更多的内容写入罗斯 - 迈尔所有的著者
A. Wauer[1]; G. I. Stangl[1]; M. Kirchgessner[1]; W. Erhardt[2]; J. Henke[2]; U. Hennig[3] and D. A. Roth-Maier[1];. A comparative evaluation of ileo-rectal anastomosis techniques for the measurement of apparent precaecal digestibilities of folate, niacin and pantothenic acid[J]. Journal of Animal Physiology and Animal Nutrition, 1999,82(2-3): 80-87