摘要
The fat mass and obesity associated (FTO) gene plays an important role in the regulation of energy homeostasis, fat deposition and obesity. For this reason, the FTO gene is a physiological and functional candidate gene for carcass and meat quality traits in beef cattle. The objectives of this study were to identify SNPs in the exonic regions of FTO gene and to evaluate the association of these SNPs with carcass traits in Hanwoo (Korean cattle). In this study, we newly identified two exonic SNPs in Hanwoo population. The g.125550A > T SNP was located in exon 3 and the g.175675C > T SNP was located in exon 6. Genotyping of the two SNP markers was carried out using PCR-RFLP analysis in Hanwoo steers to evaluate their association with carcass traits. As a result, g.125550A > T SNP genotype was significantly associated with effects on marbling score. Animals with the AA and TT homozygous genotypes had a significantly higher marbling score (p < 0.001) than those with AT heterozygous genotype, and this was significant after Bonferroni correction of the significance threshold (p = 0.003). Dominance effect was also observed for the marbling score (P < 0.05) with higher marbling score of homozygous animals. However, no significant associations with meat quality traits were observed for the g.175675C > T SNP. Our results suggest that the exonic SNP g.125550A > T in the FTO gene may be used as a DNA marker for the selection of Hanwoo with higher marbling. KeywordsFTO Marbling SNP Meat quality Hanwoo
摘要译文
关联（FTO）基因的脂肪质量和肥胖在能量稳态，脂肪沉积和obesityFor这个原因的调节中起重要作用，FTO基因对肉牛胴体和肉质性状的生理和功能候选基因这项研究的目的是在FTO基因的外显子区域，以确定的SNP，并评估这些SNP与胴体性状的HANWOO协会（韩国牛）在这项研究中，我们刚刚确定了两个单核苷酸多态性的外显子在HANWOO populationThe g550A鈥鈥涂SNP位于外显子3和g675C鈥鈥涂SNP位于外显子6这两个SNP标记基因分型进行了使用PCR-RFLP分析HANWOO操纵与胴体traitsAs因此，G来评价他们的协会125550A鈥鈥涂SNP基因型显著与机管局的大理石花纹scoreAnimals效果和TT基因型的纯合子相关有显著较高的大理石纹评分（P鈥鈥001）比那些与AT杂合基因型，这意义阈的Bonferroni校正（对鈥后是显著鈥03）也为大理石纹评分观察到的显性效应（P鈥鈥5）具有较高的大理石纹评分纯合子animalsHowever的，观察了g675C与肉质性状无显著协会鈥鈥涂SNPOur结果表明，外显子SNP摹125550A鈥鈥涂在FTO基因可以用作一个DNA标记为HANWOO的选择具有较高的大理石花纹KeywordsFTO大理石花纹的SNP肉质HANWOO
Eui-Ryong Chung [6]. Novel SNP in the coding region of the FTO gene is associated with marbling score in Hanwoo (Korean cattle)[J]. Journal of Animal Science and Technology, 2014,56(1)