摘要
IPEC-J2 cells are porcine intestinal columnar epithelial cells that were isolated from neonatal piglet mid-jejunum. This cell line forms polarized monolayers with high transepithelial electrical resistance when cultured on 0.4 μm pore-size filters. The cell line is unique in that it is derived from small intestinal tissue (compared to the common human colon-derived lines HT-29, T84, and Caco-2) and is not transformed (compared to the porcine small intestinal line, IPI-2I). Porcine intestinal epithelial cells more closely mimic human physiology than analogous rodent cell lines (e.g. IEC-6 or IEC-18), which is important in studies of zoonotic infections; in addition, they provide specificity to study porcine-derived infections. IPEC-J2 cells are increasingly being used in microbiological studies to examine the interactions of various animal and human pathogens, including Salmonella enterica and pathogenic Escherichia coli, with intestinal epithelial cells. The IPEC-J2 cell line has also been employed in some probiotic studies, in which the cells have been used as an initial screening tool for adhesiveness and anti-inflammatory properties of the potential probiotic microorganisms. The validity of these studies is not clear as follow-up studies to assess the efficacy of the probiotics in vivo have not been published to date. The aims of this review are to provide a comprehensive overview of the microbiological studies that have been conducted with IPEC-J2 cells and a reference guide of key cellular and immune markers that have been identified in this cell line that may prove to be useful in future studies.
摘要译文
IPEC-J2细胞是猪肠道柱状上皮细胞从新生儿仔猪空肠中段分离这一点。该细胞系形成极化单层高跨上皮电阻时，在0.4培养该细胞系是独特的，因为它是从小肠组织来源（相比于常见的人类结肠癌来源的系HT-29，T84，和的Caco-2）和未转化的（相比于猪小肠粘膜线，IPI-2I）。猪小肠上皮细胞更紧密地模仿人类生理比类似的啮齿动物细胞系（E。克。 IEC-6或IEC-18），这是在人畜共患感染的研究很重要;此外，他们提供的特异性来研究猪源性感染。IPEC-J2细胞正越来越多地用在微生物学研究，研究各种动物和人类病原体，包括沙门氏菌和致病性大肠杆菌的相互作用，肠上皮细胞。在IPEC-J2细胞系也被用在一些益生菌的研究，其中细胞已被用来作为一种初步筛选工具，所述潜在的益生菌微生物的粘附性和抗炎特性。这些研究的有效性是不明确的后续研究，以评估在体内益生菌的功效还没有公布最新的。已进行了用IPEC-J2细胞和已在该细胞系，可能被证明是在未来的研究有用被确定关键的细胞和免疫标记的参考指南升研究。
Amanda J. Brosnahan; David R. Brown. Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations[J]. Veterinary Microbiology, 2012,156(3-4): 229–237