摘要
The objectives were to determine the: 1) extent of epithelial and red blood cell contamination in epididymal cat sperm samples recovered by the cutting method; 2) efficacy of simple washing, single-layer centrifugation (SLC), and swim-up for selecting epididymal cat sperm; and 3) effects of freezing and thawing on cat sperm selected by various techniques. Ten unit samples were studied; each contained sperm from the cauda epididymides of four cats (total, ∼200 × 106 sperm) and was equally allocated into four treatments: 1) simple washing, 2) single-layer centrifugation through colloid prior to cryopreservation (SLC-PC), 3) single-layer centrifugation through colloid after cryopreservation (SLC-AC), and 4) swim-up. Centrifugation (300 × g for 20 min) was done for all methods. The SLC-PC had a better recovery rate than the SLC-AC and swim-up methods (mean ± SD of 16.4 ± 8.7, 10.7 ± 8.9, and 2.3 ± 1.7%, respectively; P < 0.05). The SLC-PC, SLC-AC and swim-up samples contained less red blood cell contamination than simple washed samples (0.02 ± 0.01, 0.02 ± 0.04, 0.03 ± 0.04, and 0.44 ± 0.22 × 106 cells/mL, respectively; P < 0.05). Although the proportion of sperm with head abnormalities did not differ among selection methods (P > 0.05), SLC-PC yielded the highest percentage of sperm with normal midpieces and tails (P < 0.05), due to the lowest proportion of coiled tails (P < 0.05). Furthermore, the SLC-PC was as effective as swim-up in removing sperm with proximal droplets, and selecting motile sperm, as well as those with intact membranes and DNA (P > 0.05). In conclusion, both SLC-PC and swim-up improved the quality of epididymal cat sperm, including better morphology, membrane and DNA integrity, and removal of cellular contamination. However, SLC had a better sperm recovery rate than swim-up.
摘要译文
的目的是确定：上皮细胞和由切割方法回收附睾猫精子样本中的红血细胞污染的1）的程度;简单的冲洗2）功效，单层离心（SLC）和向上游动选择附睾精子的猫;和3）选择通过各种技术冻融对猫精子的影响。十个单位的样品进行了研究;从四只猫马尾附睾包含的每个精子（总，2）通过胶体之前冷冻保存（SLC-PC的单层离心），通过胶体3）单层离心冻存（SLC-AC）后，4）游泳行动。离心（300×g的20分钟）中的所有方法进行。该SLC-PC有一个更好的恢复速度比SLC-AC和向上游动方法（平均值±16.4±8.7 SD，10.7±8.9和2.3±1.7％，分别; P22×10 SUP 6 / SUP细胞/ mL，分别为; PSLC-PC产生的精子与正常midpieces和尾巴的比例最高（P的SLC-PC一样有效滨与近端液滴除去的精子，并选择活动精子，以及那些具有完整的膜和DNA（P两个SLC-PC和游泳行动改善附睾猫精子的质量，包括更好的形态，膜和DNA完整性，并除去细胞污染。然而，SLC有一个更好的精子回收率比池畔。
K. Chatdarong[a]; P. Thuwanut[a][b]; J.M. Morrell[b]. Single-layer centrifugation through colloid selects improved quality of epididymal cat sperm[J]. Theriogenology, 2010,73(9): 1284–1292