期刊文献

Determination of semen characteristics and sperm cell ultrastructure of captive coatis (Nasua nasua) collected by electroejaculation 收藏

精液特性和圈养coatis(Nasua nasua)精子细胞超微结构测定收集electroejaculation
摘要
Despite the wide geographical distribution of coati (Nasua nasua) from the south of Canada to the north of Argentina, studies regarding the reproductive characteristics of this species are extremely limited. The objective of this study was to describe the various characteristics of coati semen by morphometric and ultrastructural analysis. Five mature males were anesthetized and electroejaculated for the collection of semen. Semen was immediately evaluated for color, volume, pH, sperm motility, vigor, morphology, acrosomal integrity, percentage of live cells and hypo-osmotic response by light microscopy. Sperm cell morphometry and ultrastructural analyses were also performed. Observations of seminal characteristics determined by electroejaculation in captive coatis represent a valuable baseline dataset for establishing fertility standards and provide background information that may be useful for assisted breeding programmes in members of the Procyonidae family.
摘要译文
尽管浣熊的地理分布广(Nasua nasua)从加拿大南部到阿根廷北部,关于该物种的繁殖特性的研究极为有限。本研究的目的是通过形态学和超微结构分析来描述浣熊精液的各种特性。五成熟雄性麻醉并electroejaculated精液的集合。精液立即评估色,量,pH值,精子活力,活力,形态,顶体完整,活细胞和光镜低渗响应的百分比。还进行了精子细胞的形态和超微结构分析。由electroejaculation圈养coatis确定精液特征观测点代表一个有价值的基准数据集,用于建立生育标准,并提供​​可能对在浣熊科家族的成员辅助的育种程序有用的背景信息。
Gabriela L. Lima; Felipe F.P.C. Barros; Leonardo L.M. Costa; Thibério S. Castelo; José D. Fontenele-Neto; Alexandre R. Silva. Determination of semen characteristics and sperm cell ultrastructure of captive coatis (Nasua nasua) collected by electroejaculation[J]. Animal Reproduction Science, 2009,115(1-4): 225–230