摘要
The objective was to compare the effects of ketamine–xylazine or tiletamine–zolazepam combinations as anesthetic protocols for captive coatis (Nasua nasua) for semen collection by electroejaculation. Five mature male coatis were physically restrained and then anesthetized by im injections of ketamine (10 mg/kg) plus xylazine (1 mg/kg) or a tiletamine–zolazepam combination (8 mg/kg). For the two combinations, additional quarter-doses of ketamine or the tiletamine–zolazepam combination were administered when necessary. Semen was collected by electroejaculation and immediately evaluated for color, volume, pH, sperm motility, vigor, morphology, acrosomal integrity, and percentage of live cells. Overall, collection of nine ejaculates was attempted from five animals for each treatment. Regardless of the anesthetic combination, all animals developed an erection during each attempt to collect semen. Ejaculates were obtained in all (9 of 9) attempts that used ketamine–xylazine for anesthesia, but in only 3 of 9 attempts (P < 0.05) when tiletamine–zolazepam was used. All ejaculates contained sperm, with no significant differences in semen characteristics between the two anesthetic combinations. Recovery was smooth in all animals. In conclusion, semen collection by electroejaculation in coatis was significantly more successful with the use of a ketamine–xylazine combination than with tiletamine–zolazepam.
摘要译文
的目的是比较的氯胺酮 - 甲苯噻嗪或替来他明 - 唑拉西泮的组合作为用于圈养coatis（Nasua nasua）精液采集由electroejaculation麻醉协议的影响。五成熟的男性coatis是物理克制，然后氯胺酮肌肉注射（10毫克/千克）加甲苯噻嗪（1毫克/千克）或替来他明 - 唑拉西泮组合（8毫克/千克）麻醉。为两个组合，产生额外四分之一剂量的氯胺酮或替来他明 - 唑拉西泮组合施用时必需的。精液收集electroejaculation并立即评估色，量，pH值，精子活力，活力，形态，顶体完整，活细胞的百分比。总体而言，收集九精液是从五畜为每个处理尝试。不管麻醉剂的组合，所有的动物每个试图收集精液中发展勃起。射精是在使用氯胺酮，甲苯噻嗪麻醉尝试所有（9 9）获得的，但只在3月9日的尝试（P所有的精液精子所含，两者麻醉组合之间的精液中无特点的差异显著。回收率在所有的动物顺利。结论，精液采集由electroejaculation在coatis是显著与使用氯胺酮 - 赛拉嗪的组合比与替来他明 - 唑拉西泮更成功。
F.F.P.C. Barros[a]; J.P.A.F. Queiroz[a]; A.C.M. Filho[b]; E.A.A. Santos[a]; V.V. Paula[a]; C.I.A. Freitas[a]; A.R. Silva[a]. Use of two anesthetic combinations for semen collection by electroejaculation from captive coatis (Nasua nasua)[J]. Theriogenology, 2009,71(8): 1261–1266