摘要
A simultaneous saccharification fermentation (SSF) system was studied for ethanol production in flour industrial sweetpotato (ISP) feedstocks (lines: white DM02-180 and purple NC-413) as an integrated cost saving process, and to examine the feasibility of extracting anthocyanins from flour purple ISPs under a simultaneous extraction and fermentation (SEF) system. Furthermore, a separate hydrolysis fermentation (SHF) configuration was carried out to establish a baseline in sugar consumption and ethanol production from the ISP lines. The thermotolerant ethanol producing yeast strain Kluyveromyces marxianus NCYC 851 and the mesophilic Saccharomyces cerevisiae Ethanol Red were evaluated, using commercial alpha amylases for hydrolysis of available ISP starch to sugars. Fermentation by S. cerevisiae during SHF had an ethanol yield of 0.32 g/g dry ISP, a 1.1-fold increase above that produced by K. marxianus. Subsequent studies showed that ethanol yield could be increased in a SSF system with a maximum ethanol yield of 0.39 g/g dry ISP achieved, a 15% increase compared with using a SHF system when S. cerevisiae was used. Simultaneous extraction and fermentation of flour purple-fleshed NC-413 ISPs was studied to evaluate the effect of pH on extraction of total monomeric anthocyanins (TMA) and ethanol production. On average, maximum ethanol yield ranged from 0.31 to 0.34 g/g dry ISP and TMA concentration ranged from 45 to 64 mg cyanidin-3-glu/100 g dry powder (10–22 mg/100 g fresh weight) with the greatest ethanol production coming from non-adjusted pH fermentations. The highest anthocyanin recovery, 64 mg cyanidin-3-glu/100 g dry powder was obtained at 35 °C and pH 4.5 using S. cerevisiae Ethanol Red. This study showed the feasibility of extracting anthocyanins and producing ethanol simultaneously in one unit operation without the need of purified solvents.
摘要译文
arification发酵（SSF）系统面粉研究了乙醇生产工业甘薯（ISP）的原料（线：白DM02-180和紫色NC-413）为一体的综合成本节约的过程，并检查一个同时提取和发酵（SEF）systemFurthermore下提取面粉紫色的ISP花青素的可行性，一个单独的水解发酵（SHF）构进行从ISP线路建立在糖消耗和乙醇生产的基线的耐热乙醇生产的酵母菌株马克斯克鲁NCYC 851和嗜温酿酒酵母乙醇红进行评价，使用商业α淀粉为SHF过程中可用的ISP淀粉sugarsFermentation由Scerevisiae水解已经0克/ g干ISP的乙醇产量，1以上，通过KmarxianusSubsequent研究产生的1倍的增加表明，乙醇产量可以在一个SSF系统实现0克/ g干的ISP的最大乙醇产量增加，25增长15％，使用一个SHF系统被用来Scerevisiae相比同时提取和面粉紫肉NC-413的ISP发酵的研究，以评估总单体花青素（TMA）和乙醇生产的提取pH值的影响平均而言，最大的乙醇产量从0℃至0范围为4g / g干的ISP和TMA浓度范围从45到以最大的乙醇产量从非调pH发酵未来64毫克矢车菊-3- GLU / 100克的干粉（10鈥毫克/ 100克鲜重）最高的花青素恢复，64毫克矢车菊素-3- GLU在35掳下获得/ 100克的干粉和pH 4sing Scerevisiae乙醇红本研究结果显示在一个单元操作同时提取花青素和生产乙醇的可行性，而无需纯化溶剂的
Joscelin T. Diaz[a]; Mari S. Chinn[a]; Van-Den Truong[b]. Simultaneous saccharification and fermentation of industrial sweetpotatoes for ethanol production and anthocyanins extraction[J]. Industrial Crops and Products, 2014,62: 53–60