摘要
Contents Leptin (LEP) and leptin receptor (LEP-R) expression was shown to change throughout the luteal phase in several species and may be involved in steroid hormone production. In the bitch, leptin but not LEP-R protein was detected in the non-pregnant corpus luteum (CL). Until now, no further information has been available on their expression levels and role in CL function. Our objective was to compare time-related changes in luteal LEP and LEP-R mRNA levels during the non-pregnant luteal phase, pregnancy and after aglepristone treatment in mid-gestation. CLs were collected by ovariohysterectomy at different time points: day (d) 5, 15, 25, 35, 45, 65 after ovulation (p.o.) in non-pregnant bitches; pre-implantation, post-implantation, mid-gestation, during prepartum luteolysis; 24 and 72 h after aglepristone injection. Non-pregnant LEP expression was lowest on d5 p.o., increased thereafter and fell again on d45 (P ≤ 0.04). LEP-R expression was not altered (P = 0.07). In pregnant bitches, neither LEP nor LEP-R mRNA levels varied over time (P = 0.201 and P = 0.150, respectively). Aglepristone treatment caused substantial downregulation of luteal LEP expression by 72h post-treatment (P ≤ 0.01). However, LEP-R expression did not follow the same course (P = 0.193). Our results indicate that both LEP and LEP-R mRNA are present in the canine CL during the non-pregnant luteal phase and pregnancy. LEP expression changes significantly over time in non-pregnant dogs and after aglepristone administration and thus, it may play a role in luteal steroidogenesis and regression.
摘要译文
内容瘦素（LEP）和瘦素受体（LEP-R）的表达被示出在整个黄体期改变几个物种，并且可以参与类固醇激素的产生。在母狗，在非妊娠黄体（CL）检测瘦素但不LEP-R蛋白。到现在为止，没有进一步的信息，已经可以在发光功能的表达水平和作用。我们的目标是在非妊娠黄体期，怀孕和在妊娠中期aglepristone处理后，比较在黄体LEP和LEP-R的mRNA水平与时间相关的变化。通函收集了卵巢子宫切除术，在不同的时间点：日（D）5，15，25，35，45，65后排卵（PO）在非怀孕的母狗;植入前，植入后，妊娠中期，在产前luteolysis; aglepristone注射后24和72小时。在D5婆非孕妇LEP表达最低，此后上升，再次倒在D45（P≤0.04）。LEP-R的表达没有改变（P \x3d 0.07）。在妊娠母犬，无论是LEP也不LEP-R mRNA水平随时间变化（P \x3d 0.201和P \x3d 0.150，分别）。Aglepristone治疗72小时后处理（P≤0.01），黄体引起的LEP表达下调可观。然而，LEP-R的表达没有按照相同的课程（P \x3d 0.193）。我们的研究结果表明，这两个LEP和LEP-R mRNA的存在于犬CL在非妊娠黄体期和妊娠。LEP表达随时间的非怀孕狗和aglepristone给药后显著变化，因此，它可能会在黄体类固醇和回归发挥作用。
O Balogh[1,*],;MP Kowalewski[2,†],;IM Reichler[1,†]. Leptin and Leptin Receptor Gene Expression in the Canine Corpus Luteum During Diestrus, Pregnancy and after Aglepristone-Induced Luteolysis[J]. Reproduction in Domestic Animals, 2012,47(Sup6): 40–42