期刊文献

Specific detection of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella from single vegetative cells by a loop-mediated isothermal amplification method 收藏

通过环介导等温扩增法特异性检测有毒甲藻亚历山大藻和链状亚历山大藻从单一营养细胞
原文求助 发布源
作者
Satoshi Nagai; Shigeru Itakura
作者单位
National Research Institute of Fisheries and Environment of Inland Sea, Maruishi 2-17-5, Hatsukaichi, Hiroshima 739-0452, Japan
关键词
Alexandrium tamarense; Alexandrium catenella; Loop-mediated isothermal amplification; Molecular detection; Plankton monitoring; Paralytic shellfish poisoning
关键词译文
亚历山大藻;链状亚历山大藻;环介导等温扩增;分子检测;浮游生物的监测;麻痹性贝类中毒
发布日期
4 April 2012
页码
43–49
DOI
10.1016/j.margen.2012.03.001
来源信息
Marine Genomics ISSN：1874-7787, 2012年, 7卷, 43–49页
摘要
In this study, we succeeded in developing a loop-mediated isothermal amplification (LAMP) method that enables sensitive and specific detection of the toxic marine dinoflagellates Alexandrium tamarense and Alexandrium catenella from single cells of both laboratory cultures and naturally blooming cells within 25 min, by monitoring the turbidimeter from the start of the LAMP reaction. The fluorescence intensity was strong enough to allow discrimination between positive and negative results by naked eye under a UV lamp, even in amplified samples from a single cell, by using the LAMP method. Unambiguous detection by naked eye was possible even in half the volume of LAMP cocktail recommended by the manufacturer, suggesting the potential to significantly reduce the cost of Alexandrium monitoring. Therefore, we can conclude that this method is one of the most convenient, sensitive, and cost-effective molecular tools for Alexandrium monitoring.
摘要译文
在这项研究中，我们成功地开发了环介​​导等温扩增（LAMP）方法，使敏感和特异的检测有毒海洋涡鞭毛藻亚历山大藻和从单细胞两者实验室培养的链状亚历山大藻和自然开花细胞内25分钟，通过从监测的LAMP反应开始的浊度仪。荧光强度是足够强，以便在UV灯下通过肉眼阳性和阴性结果之间的区别，即使在放大后的样品从单个细胞，通过利用LAMP法。明确检测由肉眼是可能的，即使在灯泡鸡尾酒的一半由制造商推荐的量，暗示潜力显著降低的亚历山大监测的成本。因此，我们可以得出结论，该方法是用于监测亚历山大最为方便，灵敏，和具有成本效益的分子的工具之一。
Satoshi Nagai; Shigeru Itakura. Specific detection of the toxic dinoflagellates Alexandrium tamarense and Alexandrium catenella from single vegetative cells by a loop-mediated isothermal amplification method[J]. Marine Genomics, 2012,7: 43–49