摘要
Abstract The L1 major capsid protein of human papillomavirus type 45 was expressed in insect cells using recombinant baculovirus technology. Human papillomavirus type 45 L1 major capsid protein self-assembled into empty virus-like particles (VLPs). These 50–60 nm diameter particles were present in the nuclei of recombinant baculovirus-infected insect cells and had a density of 1.29–1.30 g/cm3 in cesium chloride. The expressed human papillomavirus type 45 L1 protein sequence is identical to the reference human papillomavirus type 45 strain except for one amino acid located at position 49 of the human papillomavirus type 45 L1 protein. It must be noted that the quantity of purified human papillomavirus type 45 virus-like particles is at a lower level than that previously observed with human papillomavirus type 16. Nevertheless, the ability to generate preparative amounts of human papillomavirus type 45 virus-like particles is of great importance for the production of an anti-human papillomavirus vaccine. .overlined { text-decoration: overline; } .struck { text-decoration:line-through; } .underlined { text-decoration:underline; } .doubleUnderlined { text-decoration:underline;border-bottom:1px solid #000; } Get PDF (525K)Get PDF (525K) More content like thisFind more content: like this articleFind more content written by: Antoine Touzé Catherine Dupuy Martine Chabaud Pierre Le Cann Pierre Coursaget All Authors
摘要译文
人乳头瘤病毒45型摘要L1主要衣壳蛋白的表达，利用重组杆状病毒技术昆虫细胞。人乳头瘤病毒45型的L1主要衣壳蛋白的自我装配成空的病毒样颗粒（VLP）。这些50-60纳米直径的粒子存在于重组杆状病毒感染的昆虫细胞的细胞核和具有1.29-1.30克/厘米SUP 3 / SUP在氯化铯密度。llomavirus型45 L1蛋白序列是相同的参考人乳头瘤病毒45型的应变，除了一个氨基酸位于人乳头瘤病毒45型的L1蛋白的位置49。必须指出的是，纯化的人乳头瘤病毒45型病毒样颗粒的量是在一个较低的水平低于先前与人乳头瘤病毒类型16.然而观察到的，产生人乳头瘤病毒45型病毒样颗粒的制备量的能力，是用于生产抗人乳头瘤病毒疫苗非常重要的。 。上划线{文字装饰：上划线; } {.struck文字修饰：线通过; } {.underlined文字装饰：下划线; } {.doubleUnderlined文字装饰：下划线;边境底：1px的固体25K）获取PDF（525K）更多内容类似thisFind更多内容：像这样的articleFind更多的内容写的：安托万Touzé凯瑟琳·杜佩马丁CHABAUD皮埃尔乐蚕嗯皮埃尔Coursaget所有的著者
Antoine Touzé, Catherine Dupuy, Martine Chabaud, Pierre Le Cann and Pierre Coursaget[*]. Production of human papillomavirus type 45 virus-like particles in insect cells using a recombinant baculovirus[J]. FEMS Microbiology Letters, 1996,141(1): 111-116