摘要
The present study was conducted to evaluate whether supplementation of semen extender with glutathione (GSH) can maintain the quality of frozen-thawed canine spermatozoa. Eighteen ejaculates were obtained from 5 dogs and placed in extender (20% egg yolk, Tris, citric acid, lactose, raffinose, antibiotics and 6.5% glycerol) containing 0 (control), 2.5, 5, 7.5 or 10 mM GSH. The samples were cooled to 4 C and then frozen in liquid nitrogen vapor. Motility parameters of the sperm were evaluated at 0, 1, 2, 3, 4, 12 and 24 h after thawing. Sperm motility was higher in the 5 mM GSH group than in the control or 2.5 and 10 mM GSH groups; this effect was observed at 1 to 24 h after thawing (P < 0.05). The 5 mM GSH group had a higher sperm viability index at 12 and 24 h after thawing compared with the other groups (P < 0.05). Acrosome integrity, evaluated at 4 h after thawing, was greater in two of the GSH-treated groups (5 and 10 mM) compared with the control. Lipid peroxidation (LP) levels immediately after thawing were lower in the 5 and 10 mM GSH groups compared with the control, while those at 12 h after thawing did not differ significantly. Frozen-thawed semen in the 5 mM GSH group was used for transcervical insemination of 4 bitches, resulting in delivery of 5 puppies from 2 bitches. These results indicate that supplementation of semen extender with 5 mM GSH was effective in improving motility, longevity and acrosomal integrity and inhibiting LP levels in post-thaw canine spermatozoa, without any adverse impacts on full-term development after transcervical insemination.
摘要译文
本研究旨在评估精液扩展与谷胱甘肽（GSH）的补充能否维持冻融犬精子质量十八精液从5只狗得到的，并置于扩展器（20％25蛋黄，三，柠檬酸，乳糖，棉子糖，抗生素和65甘油）含有0（对照），25，75或10毫GSHThe样品冷却至4℃，然后冻结在精子的液氮vaporMotility参数在解冻后0，1，2，3，4，12和24小时，评价精子活力是越高的5mM GSH组中比对照或第二的10mM谷胱甘肽基;观察到这种效果，在1至24小时解冻后（P 在5mM的GSH组有更高的精子存活率指数在12和解冻与其他组比较24小时后（P 0Acrosome完整性，评价在4小时解冻后，与对照物更大的两个谷胱甘肽处理组（5和10mM）相比脂质过氧化（LP）的水平立即解冻后均降低，与对照相比，5和10mM GSH组，而那些在解冻后12小时也没有显著不同在5毫米GSH组冻融精子被用于4母犬宫颈人工授精，造成2母犬分娩5小狗这些结果表明的精液扩展，补充有5mM GSH的是有效地改善运动性，长寿和顶体完整，抑制解冻后精子犬LP的水平，没有宫颈人工授精后足月发展的任何不利影响
Kazuko OGATA[1) 2]; Aiko SASAKI[2) 3]; Yuka KATO[2]; Arisa TAKEDA[2]; Mikio WAKABAYASHI[3]; Borjigin SARENTONGLAGA[2) 4]; Mio YAMAGUCHI[1) 2]; Asuka HARA[1) 2]; Rika FUKUMORI[2]; Yoshikazu NAGAO[1) 2]. Glutathione supplementation to semen extender improves the quality of frozen-thawed canine spermatozoa for transcervical insemination[J]. Journal of Reproduction and Development, 2015,61(2): 116-122