摘要
The objective of this retrospective clinical study was to compare pregnancy rates obtained after the use of endoscopic-assisted transcervical catheterization (EIU) or laparotomy (SIU) for insemination of frozen-thawed dog semen. Healthy bitches from various breeds were inseminated with semen from multiple donors processed by different freezing centers. Data from 118 inseminations (78 EIU and 40 SIU) performed between 2009 and 2011 were analyzed. Insemination timing was based on vaginal cytology, serum progesterone concentrations, and vaginoscopy. A ureterorenoscope and a CH-5 Transcervical insemination catheter were used for EIU; 28 of the bitches in this group were inseminated twice with the second insemination less than 12 hours after the first. The numbers of live morphologically normal sperm (LMNS) were determined to characterize insemination doses. Overall, pregnancy rate was greater (P < 0.05) in the EIU group (65%) than in the SIU group (45%). Pregnancy rates were greater (P ≤ 0.06) when more than 100 × 106 LMNS were inseminated regardless of insemination method; the greatest pregnancy rate was observed in the EIU group when this insemination dose was used (38/49; 78%). There was no significant difference in pregnancy rate whether one (69%) or two inseminations (64%) were performed in the EIU group. Complications in the SIU group included anesthetic-induced bradycardia during surgery, significant postsurgery pain, seroma formation over the abdominal incision, and delayed wound healing. No complications were noted during or after insemination in the EIU group. In conclusion, these results support the use of EIU as a noninvasive alternative to laparotomy for insemination of frozen-thawed dog semen. In addition, use of more than 100 × 106 LMNS is also recommended for insemination.
摘要译文
spective临床研究比较使用内窥镜辅助宫腔镜插管（EIU）或剖腹（SIU）的冻融犬精液人工授精后获得的怀孕率。从各品种的健康母犬进行人工授精与来自不同冰点中心处理多个捐助者的精液。从118授精（EIU 78和40 SIU）2009年至2011年间的数据进行了分析。输精时间是根据阴道细胞学检查，血清孕酮浓度和阴道镜检查。一个ureterorenoscope和CH-5宫腔镜人工授精导管被用于EIU;该组中的雌性28分别与第二授精不少于12小时后的第一个授精的两倍。现场形态正常的精子（LMNS）的数量被确定为表征人工授精剂量。总体而言，怀孕率更高（P05）的EIU组（65％），比萧组（45％）中。怀孕率更高（P最大妊娠率观察时此授精剂量使用EIU组（38/49; 78％）。有一个人是否（69％）或两个授精（64％）是EIU组进行的妊娠率没有显著差异。萧组的并发症包括手术过程中麻醉药引起的心动过缓，显著术后疼痛，积液形成了腹壁切口，和延迟伤口愈合。期间或受精的EIU组后无并发症。结论，这些结果支持使用EIU作为一种非侵入性的替代开腹冻融犬精液人工授精。此外，使用超过100×10 SUP 6 / SUP LMNS也建议人工授精。
S.J. Mason; N.R. Rous. Comparison of endoscopic-assisted transcervical and laparotomy insemination with frozen-thawed dog semen: A retrospective clinical study[J]. Theriogenology, 2014,82(6): 844–850