摘要
Oxidative damage of sperm by means of reactive oxygen species generated by the cellular components of semen is one of the main reasons for decreased sperm motility and fertility during the freeze–thawing process. This study was conducted to determine the influence of catalase (CAT) and superoxide dismutase (SOD) on rooster sperm motility, viability and MDA level after freezing and thawing. Semen samples from 10 sexually-mature Ross 308 breeder roosters were collected and pooled, divided into nine equal parts and diluted with modified Beltsville extender containing no antioxidants (control), or supplemented with 50, 100, 200 and 300 μg/mL CAT, or 50, 100, 200 and 300 U/mL SOD. After thawing, sperm motility and motion parameters were assessed using a CASA system. Sperm viability and MDA level were assessed by eosin–nigrosin and MDA test, respectively. The results of this experiment showed that the extender supplemented with 100 and 200 μg CAT, and 50 U SOD had the highest sperm motility (P < 0.05) in sperm motility. Also, addition 100, 200 and 300 μg CAT, and 50 U SOD can improve significantly viability after freeze–thaw. Extender supplemented with 100 μg CAT had significantly lower MDA level compared to control and 300 μg CAT. In conclusion, the results of the present study demonstrate that addition of CAT (100 μg/mL) and SOD (50 U/mL) independently have beneficial effect on quality of post-thawed rooster semen.
摘要译文
通过精液的细胞成分产生的活性氧对精子造成的影响是冻融过程中精子活力和受精能力下降的主要原因之一。本研究旨在探讨过氧化氢酶（CAT）和超氧化物歧化酶（SOD）对冷冻和解冻后公鸡精子活力，活力和MDA含量的影响。收集10只性成熟的Ross 308育种雄鸡的精液样品并汇集，分成9等份，并用不含抗氧化剂（对照）的改良Beltsville填充剂稀释，或者补充50,100,200和300μg/ mL CAT或50,100,200和300U / mL SOD。解冻后，使用CASA系统评估精子活力和运动参数。伊红 - 尼格鲁辛和MDA测定分别评估精子活力和MDA水平。本实验结果表明，补充100和200μgCAT，50U SOD的补体具有最高的精子活力（P 0.05）。而且，除此之外，200和300μgCAT，50 U SOD可以显着提高冻融后的活力。与对照和300μgCAT相比，补充有100μgCAT的增量剂具有显着更低的MDA水平。总之，本研究的结果表明，CAT（100μg/ mL）和SOD（50U / mL）的加入对解冻后公鸡精液品质有独立的影响。
Mahmood Reza Amini; Hamid Kohram; Ahmad Zare-Shahaneh; Mahdi Zhandi; Hossein Sharideh; Mohammad Mehdi Nabi. The effects of different levels of catalase and superoxide dismutase in modified Beltsville extender on rooster post-thawed sperm quality ☆[J]. Cryobiology, 2015,70(3): 226–232