摘要
Many strains of Bacillus subtilis were found to secrete several hemicellulolytic enzymes such as arabinoxylanase, galactomannanase, arabinogalactanase, etc. Chemical and enzymatic properties of certain strains of the bacterium were comparatively investigated. An arabinogalactanase was purified and obtained in a crystalline state. Its molecular weight and isoelectric point were estimated to be 3.7×104 and 8.39, respectively. The enzyme showed an optimal pH for reactions at 6.0, and was stable in a pH range of 5.0 to 9.5 at 30°C. It required no metallic ions for its activity and hydrolyzed soybean arabinogalactan, forming galactobiose as the main product. No liberation of arabinose was observed in the hydrolysate. Also, the enzyme did not attack coffee bean arabinogalactan. Some implications of the experimental results are discussed.
摘要译文
枯草芽孢杆菌多株被发现分泌多种半纤维素分解酶，如阿拉伯木聚糖，半乳甘露聚糖， arabinogalactanase等化学和细菌的某些菌株的酶学性质进行了对比研究。一个arabinogalactanase纯化和结晶状态获得。其分子量和等电点被估计为3.7×10 \u003c SUP \u003e 4 \u003c / SUP \u003e和8.39 ，分别。所述酶显示出对反应的最佳pH为6.0 ，并且是稳定在5.0至9.5的pH范围在30℃ 。它不需要金属离子的活性和水解大豆阿拉伯半乳聚糖，半乳形成为主要产品。观察到水解产物阿拉伯糖没有解放。此外，酶没有攻击咖啡豆阿拉伯半乳聚糖。对实验结果的一些影响进行了讨论。
Shigenori Emi[a]; Juichiro Fukumoto[a] ; Takehiko Yamamoto[a]. Studies of Hemicellulolytic Enzymes of Bacillus subtilis[J]. Bioscience, Biotechnology, and Biochemistry, 1971,35(12): 1891-1898