摘要
Difficulties often occur in separating closely related dinoflagellate species. In this study, the potential utility of mitochondrial cytochrome b (cob) gene sequence and mRNA editing characteristics was assessed using Prorocentrum Ehrenberg as a model. The cob sequences and the patterns of their mRNA editing were analyzed for several Prorocentrum taxa. Results revealed little difference in cob sequence and mRNA editing characteristics between geographic populations of P. minimum (Pavillard) Schiller, while a notable difference was detected between different species (P. minimum and P. micans Ehrenberg). Furthermore, these P. minimum populations consistently formed a tight cluster on phylogenetic trees inferred from cob sequences as well as mRNA editing characteristics, whereas different Prorocentrum species were well separated, with a genetic distance of 0.0042±0.0024 for the former and 0.0141±0.0012 for the latter (P<0.01; two-tailed t-test). When the analysis was applied to the case of P. donghaiense Lu et Goebel and CCMP1517 strain of P. dentatum Stein, no differences were detected between these two taxa with respect to cob mRNA editing pattern and only small differences equivalent to those between P. minimum populations were detected in terms of cob sequence. On the cob sequence- and editing-based phylogenetic trees, P. donghaiense and P. dentatum CCMP1517 consistently clustered together at a position sister to P. minimum. The results suggest that cob, combined with its mRNA editing, can potentially be a useful delineator of Prorocentrum species, and that P. donghaiense and P. dentatum CCMP1517 are most likely the same species and both are closely related to P. minimum.
摘要译文
困难往往发生在分离密切相关的甲藻种。在这项研究中，使用原甲藻作为模型细胞色素b（COB）的基因序列和mRNA的编辑特点的潜在效用进行了评估。棒序列及其mRNA编辑的模式几原甲藻类群进行了分析。结果显示在COB序列和P.最低（Pavillard）席勒的地理种群之间的基因编辑特性差别不大，而不同的物种（P.最小和P.甲藻藻）之间检测到显着的差异。此外，这些第最小群始终形成一个紧密簇上从穗轴序列以及mRNA的编辑特征推断系统发生树，而不同的原甲藻种进行了很好的分离，以0.0042±0.0024前者和0.0141±0.0012，后者的遗传距离（P当分析应用到P的情况下东海路等格贝尔和P.斯坦齿的CCMP1517株，这两个类群之间的检测方面没有差别，以COB mRNA的编辑模式，只有很小的差别等同于P之间在穗轴序列而言分别检测出的最小的人口。棒序列 - 和编辑为基础的系统发育树，东海原甲藻和P.齿CCMP1517一贯的位置妹妹P.最低聚集在一起。结果表明，穗轴，结合其的mRNA编辑，有可能成为原甲藻物种的一个有用的提取器，以及东海原甲藻和P.齿CCMP1517是最有可能的同种和两个密切相关的体育最小。
Senjie Lin[1,‡]; Huan Zhang[1] and Nianzhi Jiao[2];. POTENTIAL UTILITY OF MITOCHONDRIAL CYTOCHROME B AND ITS MRNA EDITING IN RESOLVING CLOSELY RELATED DINOFLAGELLATES: A CASE STUDY OF PROROCENTRUM (DINOPHYCEAE)1[J]. Journal of Phycology, 2006,42(3): 646-654